Immunohistochemical study of basement membrane reconstruction by an epidermis-dermis recombination experiment using cultured chick embryonic skin: induction of tenascin.
Immunohistochemical study of basement membrane reconstruction by an epidermis-dermis recombination experiment using cultured chick embryonic skin: induction of tenascin.
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使用培养的鸡胚皮肤进行表皮-真皮重组实验进行基底膜重建的免疫组织化学研究：生腱蛋白的诱导。
DOI：
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发表时间：
1992
影响因子：
3.2
通讯作者：
Hiroshi Hirano
中科院分区：
文献类型：
--
作者：
Y. Akimoto;A. Obinata;H. Endo;Hiroshi Hirano
采用免疫组化方法分析了13日龄鸡胚表皮-真皮重组皮肤在基底膜形成过程中细胞外基质成分如层粘连蛋白、IV型胶原、纤维连接蛋白和腱生蛋白的产生。通过用EDTA和/或分散酶处理将表皮和真皮彼此分离。因此，基底膜的基底层从表皮和真皮中除去。将分离的表皮覆盖在分离的真皮上，即，重组，然后在Millipore过滤器上在化学成分确定的培养基（BGJb）中培养1-7天。免疫荧光标记用于光学显微镜和HRP或胶体金标记用于电子显微镜。在2天培养物的标本中，光镜下观察到抗层粘连蛋白和抗纤连蛋白反应的阳性部位，在电子显微镜水平上，这些部位与位于半桥粒附着斑下方和附近的基底层碎片相对应。重组后7天染色模式变为连续。层粘连蛋白和纤连蛋白的荧光标记出现稍早于IV型胶原和腱生蛋白。所有四种成分主要定位于基底层。纤维连接蛋白和腱生蛋白也分布在真皮细胞外基质中。在体外诱导13日龄完整胚胎皮肤基底膜中不存在的tenascin的表达。这些结果表明，半桥粒可能在基底膜的重建中发挥重要作用，并且基底膜的各种成分在重建过程中出现的不同时间。
The production of extracellular matrix components such as laminin, Type IV collagen, fibronectin, and tenascin during the formation of basement membrane in cultured epidermis-dermis recombinant skin of 13-day-old chick embryo was analyzed immunohistochemically. The epidermis and dermis were separated from each other by treatment with EDTA and/or dispase. The basal lamina of the basement membrane was thus removed from both epidermis and dermis. The isolated epidermis was overlaid onto the isolated dermis, i.e., recombined, and then cultured for 1-7 days in a chemically defined medium (BGJb) on a Millipore filter. Immunofluorescence labeling was used for light microscopy and HRP or colloidal gold labeling for electron microscopy. In specimens from 2-day cultures, positive sites of anti-laminin and anti-fibronectin reaction were observed light microscopically as patches which, at the electron microscopic level, corresponded to fragments of the basal lamina located immediately beneath and in the vicinity of the attachment plaques of the hemidesmosomes. The staining pattern became continuous 7 days after recombination. Fluorescence labeling of laminin and fibronectin appeared somewhat earlier than that of Type IV collagen and tenascin. All of the four components were found localized primarily in the basal lamina. Furthermore, fibronectin and tenascin were also distributed in the extracellular matrix of the dermis. The expression of tenascin, which does not exist in the basement membrane of 13-day-old intact embryonic skin, was induced in vitro. These results suggest that hemidesmosomes may play an important role in the reconstruction of the basement membrane and that various components of the basement membrane appeared at different times during the reconstruction.
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影响因子：
2.7
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
通讯作者：
FURCHT, LT
兔子伤口修复过程中角膜上皮锚定结构的重新组装。
影响因子：
4.4
作者：
Gipson,IK;Spurr-Michaud,S;Tisdale,A;Keough,M
通讯作者：
Keough,M
体外模型中伤口愈合的分析：层粘连蛋白和 125 x 10(3) Mr 多肽在粘附复合物形成过程中的早期出现。
影响因子：
4
作者：
Kurpakus,MA;Stock,EL;Jones,JC
通讯作者：
Jones,JC
DOI：
10.1001/archopht.1981.03930011066017
发表时间：
1981
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Hirst,LW;Kenyon,KR;Fogle,JA;Hanninen,L;Stark,WJ
通讯作者：
Stark,WJ
使用对苯二胺封固缓冲液增强特异性免疫荧光结果。
DOI：
10.1111/1523-1747.ep12508101
发表时间：
1982
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Huff,JC;Weston,WL;Wanda,KD
通讯作者：
Wanda,KD