Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide.

Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide.
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DOI:
10.1186/s13568-015-0109-4
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Rice CV
Rice CV
中科院分区:
工程技术3区
文献类型:
--
作者:
Friedline A;Zachariah M;Middaugh A;Heiser M;Khanna N;Vaishampayan P;Rice CV

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从无尘室环境中分离的短小芽孢杆菌SAFR-032芽胞与其他芽胞形成菌相比,具有更强的抗过氧化氢、干燥、紫外线辐射和化学消毒的能力。短小芽孢杆菌SAFR-032孢子的存活需要开发更严格的消毒剂才能达到标准的洁净室灭菌做法。在这里,我们报告了一种稳定的二氧化氯为基础的杀菌剂对短小芽孢杆菌SAFR-032和枯草芽孢杆菌ATCC 6051的杀灭效果。通过暴露后CFU的测量来确定细胞的存活能力。二氧化氯对短小芽孢杆菌SAFR-032孢子的杀灭效果与过氧化氢相当或更好。这些结果表明,二氧化氯通过稳定的二氧化氯产品输送,作为一种杀灭抗过氧化氢和抗紫外线孢子的手段的有效性。本文的在线版本(doi:10.1186/s13568-0150109-4)包含补充材料,授权用户可以使用。
Bacillus pumilus SAFR-032 spores isolated from a clean room environment are known to exhibit enhanced resistance to peroxide, desiccation, UV radiation and chemical disinfection than other spore-forming bacteria. The survival of B. pumilus SAFR-032 spores to standard clean room sterilization practices requires development of more stringent disinfection agents. Here, we report the effects of a stabilized chlorine dioxide-based biocidal agent against spores of B. pumilus SAFR-032 and Bacillus subtilis ATCC 6051. Viability was determined via CFU measurement after exposure. Chlorine dioxide demonstrated efficacy towards sterilization of spores of B. pumilus SAFR-032 equivalent or better than exposure to hydrogen peroxide. These results indicate efficacy of chlorine dioxide delivered through a stabilized chlorine dioxide product as a means of sterilization of peroxide- and UV-resistant spores. The online version of this article (doi:10.1186/s13568-015-0109-4) contains supplementary material, which is available to authorized users.
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