Roles of Three HSF Domain-Containing Proteins in Mediating Heat-Shock Protein Genes and Sustaining Asexual Cycle, Stress Tolerance, and Virulence in Beauveria bassiana.
Roles of Three HSF Domain-Containing Proteins in Mediating Heat-Shock Protein Genes and Sustaining Asexual Cycle, Stress Tolerance, and Virulence in Beauveria bassiana.
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三种含有 HSF 结构域的蛋白质在介导白僵菌热休克蛋白基因和维持无性周期、胁迫耐受性和毒力中的作用
DOI：
10.3389/fmicb.2018.01677
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Zhou G;Ying SH;Hu Y;Fang X;Feng MG;Wang J
具有HSF结构域的热浪费转录因子（HSF）是真菌热蛋白（HSP）基因的调节剂，许多其他矢量热元素的媒介元素，该域响应热冲击和其他应力提示昆虫病原体BASSIANA在许多真菌中与HSF1，SFL1和SKN7的三个含HSF结构域的直接相关。级别，在激活28个HSP基因的不同家族和介质差异表达中起着重叠或相反的作用HSF1在正常和热震动条件下激活大多数HSP基因时与SFL1和SKN7不同SKN7在热休克下激活一半以上的HSP基因的角色，在某些条件下还激活了其他两个蛋白质的HSP基因。在最佳25°C时ΔSFL1和ΔHSF1中的39％。这些缺失突变体在细胞壁完整性，抗氧化活性，病毒和细胞耐受性的差异上也有所不同。 B. Bassiana对环境和托管的适应性hsf1，sfl1和skn7。
Heat-shock transcription factors (HSFs) with a HSF domain are regulators of fungal heat-shock protein (HSP) genes and many others vectoring heat-shock elements, to which the domain binds in response to heat shock and other stress cues. The fungal insect pathogen Beauveria bassiana harbors three HSF domain-containing orthologous to Hsf1, Sfl1, and Skn7 in many fungi. Here, we show that the three proteins are interrelated at transcription level, play overlapping or opposite roles in activating different families of 28 HSP genes and mediate differential expression of some genes required for asexual developmental and intracellular Na+ homeostasis. Expression levels of skn7 and sfl1 largely increased in Δhsf1, which is evidently lethal in some other fungi. Hsf1 was distinct from Sfl1 and Skn7 in activating most HSP genes under normal and heat-shocked conditions. Sfl1 and Skn7 played overlapping roles in activating more than half of the HSP genes under heat shock. Each protein also activated a few HSP genes not targeted by two others under certain conditions. Deletion of sfl1 resulted in most severe growth defects on rich medium and several minimal media at optimal 25°C while such growth defects were less severe in Δhsf1 and minor in Δskn7. Conidiation level was lowered by 76% in Δskn7, 62% in Δsfl1, and 39% in Δhsf1. These deletion mutants also showed differential changes in cell wall integrity, antioxidant activity, virulence and cellular tolerance to osmotic salt, heat shock, and UV-B irradiation. These results provide a global insight into vital roles of Hsf1, Sfl1, and Skn7 in B. bassiana adaptation to environment and host.
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影响因子：
3.7
作者：
Li G;Zhou X;Kong L;Wang Y;Zhang H;Zhu H;Mitchell TK;Dean RA;Xu JR
通讯作者：
Xu JR
影响因子：
--
作者：
Bauer, Janine;Wendland, Juergen
通讯作者：
Wendland, Juergen
影响因子：
3.3
作者：
Nelson, B;Kurischko, C;Luca, FC
通讯作者：
Luca, FC
影响因子：
5.3
作者：
GALLO, GJ;PRENTICE, H;KINGSTON, RE
通讯作者：
KINGSTON, RE
影响因子：
3.6
作者：
Nicholls S;Leach MD;Priest CL;Brown AJ
通讯作者：
Brown AJ