A monolithic single-chip point-of-care platform for metabolomic prostate cancer detection.
A monolithic single-chip point-of-care platform for metabolomic prostate cancer detection.
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DOI：
10.1038/s41378-021-00243-4
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发表时间：
2021
影响因子：
7.9
通讯作者：
Cumming DRS
中科院分区：
文献类型：
--
作者：
Annese VF;Patil SB;Hu C;Giagkoulovits C;Al-Rawhani MA;Grant J;Macleod M;Clayton DJ;Heaney LM;Daly R;Accarino C;Shah YD;Cheah BC;Beeley J;Evans TRJ;Jones R;Barrett MP;Cumming DRS
全球对可在床边或医生办公室使用的快速和具有成本效益的预后和诊断工具的需求尚未得到满足，以减少严重疾病的影响。许多癌症诊断较晚，导致昂贵的治疗和预期寿命缩短。对于前列腺癌，缺乏可靠的检测抑制了筛查项目的采用。我们报告了一个微电子点护理代谢物生物标志物测量平台，并将其用于前列腺癌检测。该平台使用一组光电探测器，可在单片集成的被动微流控通道中进行靶向、多路、比色分析，在2分钟内完成对一滴人血浆中4种代谢物的联合分析。使用l-氨基酸、谷氨酸、胆碱和肌氨酸进行初步临床研究，以训练交叉验证的随机森林算法。该系统对前列腺癌的敏感性为94%，特异性为70%，曲线下面积为0.78。该技术可以实现许多类似的分析面板，因此具有革命性的低成本，快速，即时检测的潜力。
There is a global unmet need for rapid and cost-effective prognostic and diagnostic tools that can be used at the bedside or in the doctor’s office to reduce the impact of serious disease. Many cancers are diagnosed late, leading to costly treatment and reduced life expectancy. With prostate cancer, the absence of a reliable test has inhibited the adoption of screening programs. We report a microelectronic point-of-care metabolite biomarker measurement platform and use it for prostate cancer detection. The platform, using an array of photodetectors configured to operate with targeted, multiplexed, colorimetric assays confined in monolithically integrated passive microfluidic channels, completes a combined assay of 4 metabolites in a drop of human plasma in under 2 min. A preliminary clinical study using l-amino acids, glutamate, choline, and sarcosine was used to train a cross-validated random forest algorithm. The system demonstrated sensitivity to prostate cancer of 94% with a specificity of 70% and an area under the curve of 0.78. The technology can implement many similar assay panels and hence has the potential to revolutionize low-cost, rapid, point-of-care testing.
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影响因子：
5.2
作者：
Jóźwiak P;Forma E;Bryś M;Krześlak A
通讯作者：
Krześlak A
影响因子：
5.6
作者：
Accarino, Claudio;Annese, Valerio F.;Cumming, David R. S.
通讯作者：
Cumming, David R. S.
DOI：
10.7196/samj.5768
发表时间：
2012-08-01
期刊：
SAMJ: South African Medical Journal
影响因子：
--
作者：
Bohm, L;Serafin, A M;Harvey, J
通讯作者：
Harvey, J
DOI：
10.1109/tbcas.2015.2487603
发表时间：
2016-06-01
影响因子：
5.1
作者：
Cheah, Boon Chong;Macdonald, Alasdair Iain;Cumming, David R. S.
通讯作者：
Cumming, David R. S.
DOI：
10.1016/0265-928x(89)80033-1
发表时间：
1989-01-01
期刊：
BIOSENSORS
影响因子：
--
作者：
KIMURA, J;KAWANA, Y;KURIYAMA, T
通讯作者：
KURIYAMA, T