Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2.
Distinct populations of GABAergic neurons in mouse rhombomere 1 express but do not require the homeodomain transcription factor PITX2.
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DOI：
10.1016/j.mcn.2011.08.011
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发表时间：
2012-01
影响因子：
3.5
通讯作者：
Martin, Donna M.
中科院分区：
文献类型：
--
作者：
Waite, Mindy R.;Skaggs, Kaia;Kaviany, Parisa;Skidmore, Jennifer M.;Causeret, Frederic;Martin, James F.;Martin, Donna M.
后脑菱形球1 （r1）位于发育中的小鼠大脑中关键组织区峡部的尾侧和菱形球2的吻侧。r1背侧的细胞产生小脑、蓝斑和几个脑干核，而r1腹侧的细胞则产生滑车核和三叉神经核以及中缝背的血清素能和氨基丁酸能神经元。最近的研究已经确定了几个控制背r1发育的分子事件。相比之下，对于腹侧r1基因的表达和调控其形成的遗传机制知之甚少。在腹侧r1中发现了具有不同神经递质表型的神经元，包括gaba能神经元、血清素能神经元和胆碱能神经元。在这里，我们发现PITX2在小鼠胚胎腹侧r1中标记了一个独特的gaba能神经元群体。即使在缺乏PITX2的情况下，该人群似乎也保留了其gaba能身份。我们提供了一个全面的标记图，将这些pitx2阳性的gaba能神经元放置在r1区域，该区域与背缝相交并可能与背缝通信。
Hindbrain rhombomere 1 (r1) is located caudal to the isthmus, a critical organizer region, and rostral to rhombomere 2 in the developing mouse brain. Dorsal r1 gives rise to the cerebellum, locus coeruleus, and several brainstem nuclei, whereas cells from ventral r1 contribute to the trochlear and trigeminal nuclei as well as serotonergic and GABAergic neurons of the dorsal raphe. Recent studies have identified several molecular events controlling dorsal r1 development. In contrast, very little is known about ventral r1 gene expression and the genetic mechanisms regulating its formation. Neurons with distinct neurotransmitter phenotypes have been identified in ventral r1 including GABAergic, serotonergic, and cholinergic neurons. Here we show that PITX2 marks a distinct population of GABAergic neurons in mouse embryonic ventral r1. This population appears to retain its GABAergic identity even in the absence of PITX2. We provide a comprehensive map of markers that places these PITX2-positive GABAergic neurons in a region of r1 that intersects and is potentially in communication with the dorsal raphe.
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