Amplification of RNA and DNA specific for erb B in unbalanced 1;7 chromosomal translocation associated with myelodysplastic syndrome
Amplification of RNA and DNA specific for erb B in unbalanced 1;7 chromosomal translocation associated with myelodysplastic syndrome
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与骨髓增生异常综合征相关的不平衡 1;7 染色体易位中 erb B 特异性 RNA 和 DNA 的扩增
DOI：
10.1002/jcb.240320104
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发表时间：
1986
影响因子：
4
通讯作者：
R. Ash
中科院分区：
文献类型：
--
作者：
G. Woloschak;G. Dewald;R. Bahn;R. Kyle;P. Greipp;R. Ash
先前的工作已经确定了在某些治疗相关的骨髓增生异常疾病中存在不平衡的染色体异常[+der（1），t（1;7）（p11;p11）]。最近发现EGF受体位于7 p11。使用特异性的er B B癌基因，它编码的EGF受体的截短形式的探针，我们检查了RNA和DNA来自骨髓和外周血单核细胞从骨髓增生异常综合征（MDS）和急性淋巴细胞白血病（ALL），所有轴承异常克隆在他们的骨髓与类似的不平衡1：7易位。与5′-32 p标记的细胞RNA进行的DNA过量狭缝印迹杂交显示，与正常对照相比，3例MDS患者外周血和骨髓细胞中er B B特异性mRNA的积累增加了10 - 30倍。此外，在一些细胞中检测到H-ras mRNA积累的增强，但未发现其他基因如肌动蛋白、N-ras、myc、src、B-lym和其他20种基因的表达增强。在其他血液系统疾病如慢性淋巴细胞白血病、霍奇金病或淋巴瘤患者的单核细胞中，erB B表达增加不明显。对3例1：7易位不平衡的MDS患者的限制性酶切DNA进行Southern印迹分析，结果显示，外周血白色细胞中的erB B基因扩增至少20倍，而肌动蛋白杂交水平与对照组相当。ALL患者中没有明显的这种扩增。我们的数据表明，+der（1），t（1;7）（p11;p11）染色体异常可能与er B B DNA和RNA序列的扩增特异性相关。
Previous work has established the presence of an unbalanced chromosome abnormality [+der(1),t(1;7)(p11;p11)] in some therapy‐associated myelodysplastic disorders. Recently the EGF receptor has been found to reside at 7p11. Using a probe specific for erb B oncogene, which encodes a truncated form of the EGF receptor, we examined RNA and DNA derived from bone marrow and peripheral blood mononuclear cells from three patients with myelodysplastic syndromes (MDS) and one with acute lymphocytic leukemia (ALL), all bearing an abnormal clone in their bone marrow with a similar unbalanced 1;7 translocation. DNA‐excess slot blot hybridization to 5′‐32p‐labeled cellular RNA revealed from ten‐ to thirtyfold enhancement in accumulation of mRNA specific for erb B in both peripheral blood and bone marrow cells of the three MDS patients when compared to normal controls. In addition, enhancement of H‐ras mRNA accumulation was detected in some, though expression of other genes such as actin, N‐ras, myc, src, B‐lym, and 20 other genes was not found to be enhanced. Increased erb B expression was not apparent in mononuclear cells from patients with other hematologic disorders such as chronic lymphocytic leukemia, Hodgkin's disease, or lymphoma. Southern blot analysis of restriction‐enzyme‐cleaved DNA from three MDS patients with an unbalanced 1;7 translocation revealed that erb B gene was amplified at least twentyfold in peripheral blood white blood cells, while levels of actin hybridization were comparable to those of the controls. No such amplification was evident in the ALL patient. Our data suggest that +der(1),t(1;7)(p11;p11) chromosomal anomalies can be specifically associated with amplification of erb B DNA and RNA sequences.
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DOI：
10.1016/s0021-9258(17)43493-4
发表时间：
1984-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Mariani;R. Schimke
通讯作者：
B. Mariani;R. Schimke
与小鼠逆转录病毒样多基因家族互补的多聚腺苷酸化RNA可通过表皮生长因子刺激静止细胞而快速且特异性地诱导。
DOI：
10.1073/pnas.79.23.7317
发表时间：
1982
影响因子：
11.1
作者：
Foster,DN;Schmidt,LJ;Hodgson,CP;Moses,HL;Getz,MJ
通讯作者：
Getz,MJ
影响因子：
56.9
作者：
SLAMON, DJ;DEKERNION, JB;CLINE, MJ
通讯作者：
CLINE, MJ
易位 (1;7)(p11;p11)：一种新的骨髓增殖性血液学实体。
影响因子：
--
作者：
Sandberg,AA;Morgan,R;Hecht,BK;Hecht,F
通讯作者：
Hecht,F