Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa.
Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa.
复制标题
镁和钙过于积聚在脑脑的Schengen3突变体的叶片中。
DOI：
10.1093/plphys/kiab150
复制
发表时间：
2021-07-06
期刊：
影响因子：
7.4
通讯作者：
Broadley MR
中科院分区：
文献类型：
--
作者：
Alcock TD;Thomas CL;Ó Lochlainn S;Pongrac P;Wilson M;Moore C;Reyt G;Vogel-Mikuš K;Kelemen M;Hayden R;Wilson L;Stephenson P;Østergaard L;Irwin JA;Hammond JP;King GJ;Salt DE;Graham NS;White PJ;Broadley MR
镁（Mg）和钙（Ca）是许多人类食物系统中缺乏的必需矿物质营养素。在放牧牲畜中，镁和钙缺乏是代价高昂的福利问题。在这里，我们报道了一个油菜功能缺失申根3 （sgn3）突变体braA.sgn3。a-1，它在叶片中积累了两倍的镁和三分之一的钙。我们映射了braA.sgn3。利用化学诱变系的正向基因组筛选，随后进行回交，并对第二回交的第二代子代（BC2F2）分离进行连锁阅读测序。共聚焦成像显示根内胚层扩散屏障被破坏，这与在拟南芥（拟南芥）中报道的SGN3编码Casparian条带正常形成所需的受体样激酶一致。元素空间分布分析表明，braA.sgn3叶片胞外Mg浓度升高。a-1，假设是由于过量的Mg从细胞优先输出，以确保适当的细胞浓度。这项工作证实了SGN3在控制油菜营养平衡中的保守作用，并揭示了植物能够处理由“泄漏”的根内胚层屏障引起的茎部元素浓度紊乱的机制。在诱变的rapa种群中，叶片Mg和Ca积累变化的特征表明，在育种计划中利用这些种群来增加人类和动物必需营养物质的可食用浓度是有希望的。“漏”根Casparian条导致油菜突变体叶片中镁含量增加两倍，钙含量增加约35%。
Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) mutant, braA.sgn3.a-1, which accumulates twice as much Mg and a third more Ca in its leaves. We mapped braA.sgn3.a to a single recessive locus using a forward ionomic screen of chemically mutagenized lines with subsequent backcrossing and linked-read sequencing of second back-crossed, second filial generation (BC2F2) segregants. Confocal imaging revealed a disrupted root endodermal diffusion barrier, consistent with SGN3 encoding a receptor-like kinase required for normal formation of Casparian strips, as reported in thale cress (Arabidopsis thaliana). Analysis of the spatial distribution of elements showed elevated extracellular Mg concentrations in leaves of braA.sgn3.a-1, hypothesized to result from preferential export of excessive Mg from cells to ensure suitable cellular concentrations. This work confirms a conserved role of SGN3 in controlling nutrient homeostasis in B. rapa, and reveals mechanisms by which plants are able to deal with perturbed shoot element concentrations resulting from a “leaky” root endodermal barrier. Characterization of variation in leaf Mg and Ca accumulation across a mutagenized population of B. rapa shows promise for using such populations in breeding programs to increase edible concentrations of essential human and animal nutrients. “Leaky” root Casparian strips lead to accumulation of two-fold greater magnesium and ∼35% greater calcium concentrations in leaves of a Brassica rapa mutant.
登录
查看更多内容
影响因子：
5.6
作者：
Borpatragohain P;Rose TJ;King GJ
通讯作者：
King GJ
影响因子：
6.9
作者：
Broadley, MR;Bowen, HC;White, PJ
通讯作者：
White, PJ
影响因子：
1.3
作者：
Kump, P.;Vogel-Mikus, K.
通讯作者：
Vogel-Mikus, K.
DOI：
10.1073/pnas.0910772107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Alassimone, Julien;Naseer, Sadaf;Geldner, Niko
通讯作者：
Geldner, Niko
影响因子：
4.6
作者：
Kumssa DB;Joy EJ;Ander EL;Watts MJ;Young SD;Walker S;Broadley MR
通讯作者：
Broadley MR