Characterization of a newly developed chicken 44K Agilent microarray.
Characterization of a newly developed chicken 44K Agilent microarray.
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DOI：
10.1186/1471-2164-9-60
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发表时间：
2008-01-31
期刊：
影响因子：
4.4
通讯作者：
Zhou H
中科院分区：
文献类型：
--
作者：
Li X;Chiang HI;Zhu J;Dowd SE;Zhou H
微阵列技术的发展大大提高了我们评估基因表达的能力。理论上，可以同时监测给定生物体中所有基因的表达。鸡基因组测序为设计一个全面的鸡转录组芯片提供了重要信息。一个长的寡核苷酸微阵列已经手动策划和设计，由我们的小组和制造使用安捷伦喷墨技术。这为基因表达研究提供了一个灵活而强大的平台，具有高灵敏度和特异性。鸡60-mer寡核苷酸微阵列由42，034个特征组成，包括整个马立克氏病病毒，两种禽流感病毒（H5 N2和H5 N3）和150个鸡microRNA。在一项重要的验证研究中，将从四种主要鸡组织（盲肠扁桃体（C）、回肠（I）、肝脏（L）和脾脏（S））中分离的总RNA用于比较杂交。95%以上的斑点具有高信噪比（SNR > 10）。结果表明，肝与脾、脾与盲肠扁桃体、盲肠扁桃体与回肠、肝与盲肠扁桃体、肝与回肠、脾与回肠之间差异表达的基因分别为2886、2660、358、3208、3355和3710条（P < 10-7）。在盲肠扁桃体、回肠、肝脏和脾脏中发现了大量的组织选择性基因（分别为95、71、535和108; P < 10-7）。这些数据的另一个亮点揭示了与其他测试组织相比，抗微生物肽GAL 1、GAL 2、GAL 6和GAL 7在脾脏中高度表达。设计了鸡60-mer寡核苷酸44 K微阵列，并在不同组织中基因表达的全面调查中进行了验证。组织表达谱分析结果表明，该基因芯片具有较高的特异性和敏感性，将为鸡功能基因组学研究提供有用的工具。新的数据表达的推定组织特异性基因和抗菌肽的突出显示，作为这一全面的微阵列验证研究的一部分。有关访问和订购此44 K chicken数组的信息，请访问
The development of microarray technology has greatly enhanced our ability to evaluate gene expression. In theory, the expression of all genes in a given organism can be monitored simultaneously. Sequencing of the chicken genome has provided the crucial information for the design of a comprehensive chicken transcriptome microarray. A long oligonucleotide microarray has been manually curated and designed by our group and manufactured using Agilent inkjet technology. This provides a flexible and powerful platform with high sensitivity and specificity for gene expression studies. A chicken 60-mer oligonucleotide microarray consisting of 42,034 features including the entire Marek's disease virus, two avian influenza virus (H5N2 and H5N3), and 150 chicken microRNAs has been designed and tested. In an important validation study, total RNA isolated from four major chicken tissues: cecal tonsil (C), ileum (I), liver (L), and spleen (S) were used for comparative hybridizations. More than 95% of spots had high signal noise ratio (SNR > 10). There were 2886, 2660, 358, 3208, 3355, and 3710 genes differentially expressed between liver and spleen, spleen and cecal tonsil, cecal tonsil and ileum, liver and cecal tonsil, liver and ileum, spleen and ileum (P < 10-7), respectively. There were a number of tissue-selective genes for cecal tonsil, ileum, liver, and spleen identified (95, 71, 535, and 108, respectively; P < 10-7). Another highlight of these data revealed that the antimicrobial peptides GAL1, GAL2, GAL6 and GAL7 were highly expressed in the spleen compared to other tissues tested. A chicken 60-mer oligonucleotide 44K microarray was designed and validated in a comprehensive survey of gene expression in diverse tissues. The results of these tissue expression analyses have demonstrated that this microarray has high specificity and sensitivity, and will be a useful tool for chicken functional genomics. Novel data on the expression of putative tissue specific genes and antimicrobial peptides is highlighted as part of this comprehensive microarray validation study. The information for accessing and ordering this 44K chicken array can be found at
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影响因子：
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