Cyclic AMP‐Dependent Protein Phosphorylation in Chemosensory Neurons: Identification of Cyclic Nucleotide‐Regulated Phosphoproteins in Olfactory Cilia
Cyclic AMP‐Dependent Protein Phosphorylation in Chemosensory Neurons: Identification of Cyclic Nucleotide‐Regulated Phosphoproteins in Olfactory Cilia
复制标题
化学感应神经元中环 AMP 依赖性蛋白磷酸化：嗅觉纤毛中环核苷酸调节磷酸蛋白的鉴定
作者：
Judith Heldman;D. Lancet
摘要化学感受性树突状细胞膜（嗅纤毛）含有蛋白激酶活性，该活性可被环腺苷酸（cAMP）刺激，更有效的是被不可水解的GTP类似物鸟苷-5 ′-O-（3-硫代）三磷酸（GTPγS）刺激。在对照非感觉（呼吸）纤毛中，环AMP依赖性蛋白激酶实际上对GTPγS不敏感。嗅觉酶的GTPγS激活似乎是由刺激性GTP结合蛋白（G蛋白）和腺苷酸环化酶介导的，先前显示这些蛋白在感觉膜中富集。在测试条件下，在化学感受纤毛制剂中不能检测到蛋白激酶C活性。嗅纤毛与[γ-32 P]ATP孵育导致[32 P]磷酸掺入许多多肽中，其中四种多肽以环核苷酸依赖性方式进行共价修饰。一种多肽pp 24的磷酸化在浓度低于1 μM时被环AMP强烈且特异性地增强。这种磷蛋白不存在于呼吸纤毛中，但也见于去除纤毛后从嗅神经上皮制备的膜中。环AMP依赖性蛋白激酶和磷蛋白pp 24可能是转导气味信号的分子机制的候选成分。
Abstract Chemosensory dendritic membranes (olfactory cilia) contain protein kinase activity that is stimulated by cyclic AMP and more efficiently by the nonhydrolyzable GTP analog guanosine‐5′‐O‐(3‐thio)triphosphate (GTPγS). In control nonsensory (respiratory) cilia, the cyclic AMP‐dependent protein kinase is practically GTPγS‐insensitive. GTPγS activation of the olfactory enzyme appears to be mediated by a stimulatory GTP‐binding protein (G‐pro‐tein) and adenylate cyclase previously shown to be enriched in the sensory membranes. Protein kinase C activity cannot be detected in the chemosensory cilia preparation under the conditions tested. Incubation of olfactory cilia with [γ‐32P]ATP leads to the incorporation of [32P]phosphate into many polypeptides, four of which undergo covalent modification in a cyclic nucleotide‐dependent manner. The phosphorylation of one polypeptide, pp24, is strongly and specifically enhanced by cyclic AMP at concentrations lower than 1 μM. This phosphoprotein is not present in respiratory cilia, but is seen also in membranes prepared from olfactory neuroepithelium after cilia removal. Cyclic AMP‐dependent protein kinase and phosphoprotein pp24 may be candidate components of the molecular machinery that transduces odor signals.
嗅觉 GTP 结合蛋白：脊椎动物化学感应神经元的信号转导多肽。
DOI：
10.1073/pnas.83.13.4947
发表时间：
1986
影响因子：
11.1
作者：
Pace,U;Lancet,D
通讯作者：
Lancet,D
DOI：
10.1126/science.6474180
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Nestler,EJ;Walaas,SI;Greengard,P
通讯作者：
Greengard,P