Avian auditory hair cell regeneration is accompanied by JAK/STAT-dependent expression of immune-related genes in supporting cells.
Avian auditory hair cell regeneration is accompanied by JAK/STAT-dependent expression of immune-related genes in supporting cells.
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DOI：
10.1242/dev.200113
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发表时间：
2022-04-15
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影响因子：
--
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鸟类的听觉器官是基底乳头，与哺乳动物的耳蜗形成鲜明对比的是，它可以再生感觉毛细胞，从而在几周内从耳聋中恢复过来。在体内触发、维持和终止再生反应的机制在很大程度上是未知的。在这里，我们利用rna测序分析了氨基糖苷类抗生素诱导的毛细胞丢失后鸡基底乳头基因表达的变化。我们发现了一组免疫相关基因表达的变化，并通过单细胞rna测序证实了这些变化发生在支持细胞中。原位杂交被用来进一步验证这些发现。我们确定JAK/STAT信号通路在诱导毛细胞脱落后第二天支持细胞中损伤反应基因的上调中是必不可少的。耳毒性损伤后4天，我们发现新生再生的听觉毛细胞表达与终止JAK/STAT信号应答相关的基因。支持细胞中免疫相关基因的稳定、短暂表达表明JAK/STAT信号在感觉毛细胞再生中的潜在功能参与。摘要：鸟内耳兼性干细胞在耳毒性药物诱导的毛细胞丢失后的基因表达变化。
The avian hearing organ is the basilar papilla that, in sharp contrast to the mammalian cochlea, can regenerate sensory hair cells and thereby recover from deafness within weeks. The mechanisms that trigger, sustain and terminate the regenerative response in vivo are largely unknown. Here, we profile the changes in gene expression in the chicken basilar papilla after aminoglycoside antibiotic-induced hair cell loss using RNA-sequencing. We identified changes in gene expression of a group of immune-related genes and confirmed with single-cell RNA-sequencing that these changes occur in supporting cells. In situ hybridization was used to further validate these findings. We determined that the JAK/STAT signaling pathway is essential for upregulation of the damage-response genes in supporting cells during the second day after induction of hair cell loss. Four days after ototoxic damage, we identified newly regenerated, nascent auditory hair cells that express genes linked to termination of the JAK/STAT signaling response. The robust, transient expression of immune-related genes in supporting cells suggests a potential functional involvement of JAK/STAT signaling in sensory hair cell regeneration. Summary: Gene expression changes in the facultative stem cells of the avian inner ear after ototoxic drug-induced hair cell loss in vivo.
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影响因子：
8.8
作者：
Benkafadar，Nesrine;Janesick，Amanda;Heller，Stefan
通讯作者：
Heller，Stefan
影响因子：
2.5
作者：
Cafaro, Jon;Lee, Gi Soo;Stone, Jennifer S.
通讯作者：
Stone, Jennifer S.
影响因子：
2.8
作者：
Hirose K;Rutherford MA;Warchol ME
通讯作者：
Warchol ME
影响因子：
5.3
作者：
Dinh CT;Goncalves S;Bas E;Van De Water TR;Zine A
通讯作者：
Zine A
DOI：
10.1016/j.biocel.2009.05.007
发表时间：
2009-11-01
影响因子：
4
作者：
Camp, Aaron James;Wijesinghe, Rajiv
通讯作者：
Wijesinghe, Rajiv