Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system.
Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system.
复制标题
DOI：
10.1038/s41598-017-11533-1
复制
发表时间：
2017-09-21
影响因子：
4.6
通讯作者：
Szita N
中科院分区：
文献类型：
--
作者：
Dimov N;Kastner E;Hussain M;Perrie Y;Szita N
Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body.它们目前被用于多种纳米药物配方中。然而，它们的开发和应用仍然受到昂贵且耗时的工艺开发和生产方法的限制。 Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are needed. We present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production.该系统针对一系列脂质成分、大小和电荷不同的小囊泡（低于 300 nm）进行了评估；它提供有效和快速的纳米药物纯化，具有高脂质回收率（> 98％），并在不到4分钟的时间内有效去除非包埋药物（异丙酚减少> 95％的非包埋药物）或蛋白质（卵清蛋白> 90％减少OVA）和有机溶剂（乙醇> 95％减少）。 The key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.
Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body. They are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are needed. We present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge; it offers effective and rapid nanomedicine purification with high lipid recovery (> 98%) combined with effective removal of non-entrapped drug (propofol >95% reduction of non-entrapped drug present) or protein (ovalbumin >90% reduction of OVA present) and organic solvent (ethanol >95% reduction) in less than 4 minutes. The key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.
登录
查看更多内容
影响因子：
9.5
作者：
He, Jie;Wang, Lei;Nie, Zhihong
通讯作者：
Nie, Zhihong
影响因子：
--
作者：
O'Sullivan, Brian;Al-Bahrani, Homam;Szita, Nicolas
通讯作者：
Szita, Nicolas
影响因子：
4.1
作者：
KIRBY, C;CLARKE, J;GREGORIADIS, G
通讯作者：
GREGORIADIS, G
影响因子：
3.9
作者：
Jahn, Andreas;Vreeland, Wyatt N.;Gaitan, Michael
通讯作者：
Gaitan, Michael
DOI：
10.1038/mtna.2012.28
发表时间：
2012-08-14
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
通讯作者：
--