Spatial differences in corneal electroretinogram potentials measured in rat with a contact lens electrode array.
Spatial differences in corneal electroretinogram potentials measured in rat with a contact lens electrode array.
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DOI：
10.1007/s10633-014-9459-5
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发表时间：
2014-12
影响因子：
1.4
通讯作者：
Hetling, John R.
中科院分区：
文献类型：
--
作者：
Krakova, Yelena;Tajalli, Hadi;Thongpang, Sanitta;Derafshi, Zahra;Ban, Tamas;Rahmani, Safa;Selner, Ashley N.;Al-Tarouti, Amani;Williams, Justin C.;Hetling, John R.
几十年来，人们已经知道角膜视网膜电图（ERG）的幅度随着眼表面上的位置而变化，特别是在存在局灶性或不对称刺激或视网膜病变的情况下。然而，这种现象还没有得到很好的特点，使用同时测量在角膜上的多个位置。这项工作提供了第一个表征的空间差异，在视网膜电图在整个大鼠角膜。采用接触式透镜电极阵列记录正常视力Long-Evans大鼠在短暂全场闪光刺激后同时在25个角膜位置的ERG电位。分析这些多电极视网膜电图（meERG）反应的a波和b波振幅和隐式时间的空间差异。可以可靠地记录空间上不同的ERG电位。比较整个角膜位置的相对振幅表明，当使用全视野、近饱和刺激时，存在轻微的不均匀分布。角膜下象限的a波和b波振幅比上级象限低约3%。目前的结果包括大鼠眼睛的第一个标准meERG数据库的开始，并提供了一个基础，从眼睛的功能缺陷的结果进行比较。角膜电位的空间差异的稳健测量也将支持ERG的计算源模型的优化和验证。为了充分利用meERG数据中包含的信息，最终需要详细了解局部角膜电位的许多决定因素的作用。
It has been known for several decades that the magnitude of the corneal electroretinogram (ERG) varies with position on the eye surface, especially in the presence of focal or asymmetric stimuli or retinal lesions. However, this phenomenon has not been well-characterized using simultaneous measurements at multiple locations on the cornea. This work provides the first characterization of spatial differences in the ERG across the rat cornea. A contact lens electrode array was employed to record ERG potentials at 25 corneal locations simultaneously following brief full-field flash stimuli in normally sighted Long-Evans rats. These multi-electrode electroretinogram (meERG) responses were analyzed for spatial differences in a-wave and b-wave amplitudes and implicit times. Spatially distinct ERG potentials could be recorded reliably. Comparing relative amplitudes across the corneal locations suggested a slight non-uniform distribution when using full-field, near-saturating stimuli. Amplitudes of a- and b-waves were approximately 3 % lower in the inferior quadrant than in the superior quadrant of the cornea. The present results comprise the start of the first normative meERG database for rat eyes and provide a basis for comparison of results from eyes with functional deficit. Robust measures of spatial differences in corneal potentials will also support optimization and validation of computational source models of the ERG. To fully utilize the information contained in the meERG data, a detailed understanding of the roles of the many determinants of local corneal potentials will eventually be required.
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影响因子：
1.4
作者：
Marmor, M. F.;Fulton, A. B.;Bach, M.
通讯作者：
Bach, M.
影响因子：
3.2
作者：
DOSLAK, MJ;PLONSEY, R;THOMAS, CW
通讯作者：
THOMAS, CW
影响因子：
1.4
作者：
Lyons, Jonathan S.;Severns, Matthew L.
通讯作者：
Severns, Matthew L.
影响因子：
1.8
作者：
Salinas-Navarro, M.;Mayor-Torroglosa, S.;Vidal-Sanz, M.
通讯作者：
Vidal-Sanz, M.
影响因子：
3.2
作者：
Job, HM;Keating, D;Parks, S
通讯作者：
Parks, S