Effects of chronic calorie restriction or dietary resveratrol supplementation on insulin sensitivity markers in a primate, Microcebus murinus.
Effects of chronic calorie restriction or dietary resveratrol supplementation on insulin sensitivity markers in a primate, Microcebus murinus.
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DOI：
10.1371/journal.pone.0034289
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Pifferi F
中科院分区：
文献类型：
--
作者：
Marchal J;Blanc S;Epelbaum J;Aujard F;Pifferi F
糖尿病和高胰岛素血症的患病率随着年龄的增长而增加，导致代谢衰竭并限制寿命。没有营养不良的热量限制（CR）延缓了衰老过程，但其长期应用于人类似乎很困难。白藜芦醇（RSV），一种膳食多酚，似乎是一种很有前途的CR模拟物，可以很容易地在人体内给药。在这项工作中，我们假设CR和RSV影响胰岛素敏感性在非人灵长类动物相比，标准喂养的对照（CTL）动物。将4 - 5岁的雄性灰鼠狐猴（Microcebus murinus）分为3个饮食组：CTL组，CR组，其摄入的热量比CTL少30%，RSV组，其摄入的CTL饮食中添加RSV（200 mg·day−1·kg−1）。在长期治疗21或33个月后，使用口服葡萄糖耐量试验（OGTT）和胰岛素抵抗的稳态模型评估（HOMA-IR指数）评估胰岛素敏感性和胰岛素抵抗。还测量了静息代谢率，以评估该能量消耗参数与胰岛素敏感性标志物之间的潜在关系。除了CR动物葡萄糖负荷后30分钟葡萄糖水平较低外，在21个月的治疗期后未发现差异。33个月后，CR和RSV在口服葡萄糖负荷后下降了10%，而空腹血胰岛素没有下降。观察到给药对HOMA-IR指数的一般影响，与CTL相比，给药33个月后CR动物降低81%，RSV动物降低53%。慢性CR和饮食补充RSV通过改善动物的葡萄糖耐量而不干扰其基线胰岛素分泌来影响胰岛素敏感性。这些结果表明，CR和RSV都对代谢改变具有有益作用，尽管这些作用在两种抗衰老治疗之间的幅度不同，并且可能依赖于不同的代谢变化。
The prevalence of diabetes and hyperinsulinemia increases with age, inducing metabolic failure and limiting lifespan. Calorie restriction (CR) without malnutrition delays the aging process, but its long-term application to humans seems difficult. Resveratrol (RSV), a dietary polyphenol, appears to be a promising CR mimetic that can be easily administered in humans. In this work, we hypothesized that both CR and RSV impact insulin sensitivity in a non-human primate compared to standard-fed control (CTL) animals. Four- to five-year-old male grey mouse lemurs (Microcebus murinus) were assigned to three dietary groups: a CTL group, a CR group receiving 30% fewer calories than the CTL and a RSV group receiving the CTL diet supplemented with RSV (200 mg·day−1·kg−1). Insulin sensitivity and glycemia were assessed using an oral glucose tolerance test (OGTT) and the homeostasis model assessment of insulin resistance (HOMA-IR index) evaluation after 21 or 33 months of chronic treatment. Resting metabolic rate was also measured to assess the potential relationships between this energy expenditure parameter and insulin sensitivity markers. No differences were found after a 21-month period of treatment, except for lower glucose levels 30 min after glucose loading in CR animals. After 33 months, CR and RSV decreased glycemia after the oral glucose loading without decreasing fasting blood insulin. A general effect of treatment was observed on the HOMA-IR index, with an 81% reduction in CR animals and 53% in RSV animals after 33 months of treatment compared to CTL. Chronic CR and dietary supplementation with RSV affected insulin sensitivity by improving the glucose tolerance of animals without disturbing their baseline insulin secretion. These results suggest that both CR and RSV have beneficial effects on metabolic alterations, although these effects are different in amplitude between the two anti-aging treatments and potentially rely on different metabolic changes.
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DOI：
10.1126/science.1173635
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colman RJ;Anderson RM;Johnson SC;Kastman EK;Kosmatka KJ;Beasley TM;Allison DB;Cruzen C;Simmons HA;Kemnitz JW;Weindruch R
通讯作者：
Weindruch R
影响因子：
--
作者：
KEMNITZ, JW;ROECKER, EB;BERGMAN, RN
通讯作者：
BERGMAN, RN
DOI：
10.1093/gerona/50a.6.b337
发表时间：
1995-11-01
影响因子：
5.1
作者：
CEFALU, WT;BELLFARROW, AD;THORPE, SR
通讯作者：
THORPE, SR
DOI：
10.1016/j.tem.2009.11.005
发表时间：
2010-03
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
Anderson RM;Weindruch R
通讯作者：
Weindruch R
影响因子：
4.3
作者：
Barrett, Karlene;McGrowder, Donovan;Ragoobirsingh, Dalip
通讯作者：
Ragoobirsingh, Dalip