Extensive transcriptional variation poses a challenge to thermal stress biomarker development for endangered corals
Extensive transcriptional variation poses a challenge to thermal stress biomarker development for endangered corals
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广泛的转录变异对濒危珊瑚的热应激生物标志物开发提出了挑战
DOI：
10.1111/mec.14517
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发表时间：
2018
影响因子：
4.9
通讯作者：
I. Baums
中科院分区：
文献类型：
--
作者：
J. E. Parkinson;E. Bartels;M. Devlin;C. Lustic;K. Nedimyer;S. Schopmeyer;D. Lirman;T. Lajeunesse;I. Baums
随着气候变化，对珊瑚礁生物产生负面影响的海面温度异常的频率和强度不断增加。然而，尽管珊瑚死亡率普遍较高，但即使在那些被列为受威胁的珊瑚物种中，遗传多样性仍然很高。虽然这在很多方面都是好消息，但它对开发能够识别弹性或脆弱基因型的生物标志物提出了挑战。利用佛罗里达州的三个珊瑚恢复苗圃作为长期公共花园实验，我们将 30 多个遗传上不同的鹿角鹿角菌群暴露于季节性的冷热冲击，并使用 RNAseq 测量了汇总的基因表达反应。针对 20 个基因的子集，我们设计了一个高通量 qPCR 阵列，以分别量化每次治疗下所有个体的表达，目的是识别预测和/或诊断热应激生物标志物。我们观察到种群中存在广泛的转录变异，这表明丰富的原材料可用于通过自然选择进行适应。然而，这种高度变异使得很难将基因表达变化与菌落性能指标（例如生长、死亡率和白化敏感性）关联起来。尽管如此，我们还是发现了几种有前景的急性热应激诊断生物标志物，这些标志物可能会在未来改善珊瑚恢复和减缓气候变化的努力。
As climate changes, sea surface temperature anomalies that negatively impact coral reef organisms continue to increase in frequency and intensity. Yet, despite widespread coral mortality, genetic diversity remains high even in those coral species listed as threatened. While this is good news in many ways, it presents a challenge for the development of biomarkers that can identify resilient or vulnerable genotypes. Taking advantage of three coral restoration nurseries in Florida that serve as long‐term common garden experiments, we exposed over 30 genetically distinct Acropora cervicornis colonies to hot and cold temperature shocks seasonally and measured pooled gene expression responses using RNAseq. Targeting a subset of 20 genes, we designed a high‐throughput qPCR array to quantify expression in all individuals separately under each treatment with the goal of identifying predictive and/or diagnostic thermal stress biomarkers. We observed extensive transcriptional variation in the population, suggesting abundant raw material is available for adaptation via natural selection. However, this high variation made it difficult to correlate gene expression changes with colony performance metrics such as growth, mortality and bleaching susceptibility. Nevertheless, we identified several promising diagnostic biomarkers for acute thermal stress that may improve coral restoration and climate change mitigation efforts in the future.
DOI：
10.1073/pnas.86.1.172
发表时间：
1989-01-01
影响因子：
11.1
作者：
MORSE, D;MILOS, PM;HASTINGS, JW
通讯作者：
HASTINGS, JW
影响因子：
4.1
作者：
Putnam HM;Davidson JM;Gates RD
通讯作者：
Gates RD
影响因子：
4.9
作者：
Devlin-Durante, M. K.;Miller, M. W.;Baums, I. B.
通讯作者：
Baums, I. B.