Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors.
Two types of transgenic lines for doxycycline-inducible, cell-specific gene expression in zebrafish ultraviolet cone photoreceptors.
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DOI：
10.1016/j.gep.2014.01.002
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发表时间：
2014-03
影响因子：
1.2
通讯作者：
Jensen, Abbie M.
中科院分区：
文献类型：
--
作者：
West, Megan C.;Campbell, Leah J.;Willoughby, John J.;Jensen, Abbie M.
Temporal and spatial control of gene expression is important for studying the molecular and cellular mechanisms of development, physiology, and disease. We used the doxycycline (Dox)-inducible, Tet-On System to develop transgenic zebrafish for inducible, cell specific control of gene expression in the ultraviolet (UV) cone photoreceptors. Two constructs containing the reverse tetracycline-controlled transcriptional transactivator (rtTA) gene driven by the UV opsin-specific promoter (opn1sw1) were used to generate stable transgenic zebrafish lines using the Tol2-based transgenesis method. One construct included a self-reporting GFP (opn1sw1:rtTA, TRE:GFP) and the other incorporated an epitope tag on the rtTA protein (opn1sw1:rtTAflag). UV cone-specific expression of TRE-controlled transgenes was induced by Dox treatment in larvae and adults. Induction of gene expression was observed in 96% of all larval UV cones within 16 hours of Dox treatment. UV cone-specific expression of two genes from a bidirectional TRE construct injected into one-cell Tg(opn1sw1:rtTAflag) embryos were also induced by Dox treatment. In addition, UV cone-specific expression of Crb2aIntraWT was induced by Dox treatment in progeny from crosses of the TRE-response transgenic line, Tg(TRE:HA-Crb2aIntraWT), to the Tg(opn1sw1:rtTA, TRE:GFP) line and the Tg(opn1sw1:rtTAflag) line. These lines can be used in addition to the inducible, rod-specific gene expression system from the Tet-On Toolkit to elucidate the photoreceptor-specific effects of genes of interest in photoreceptor cell biology and retinal disease.
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影响因子：
3.4
作者：
ROHLICH, P;ADAMUS, G;HARGRAVE, PA
通讯作者：
HARGRAVE, PA
影响因子：
16.2
作者：
RAYMOND, PA;BARTHEL, LK;KNIGHT, JK
通讯作者：
KNIGHT, JK
影响因子：
4.7
作者：
Le YZ;Bai Y;Zhu M;Zheng L
通讯作者：
Zheng L
影响因子：
4.3
作者：
Dooley, Christopher M.;Schwarz, Heinz;Geisler, Robert
通讯作者：
Geisler, Robert
DOI：
10.1073/pnas.92.23.10545
发表时间：
1995-11-07
影响因子：
11.1
作者：
BROCKERHOFF, SE;HURLEY, JB;DOWLING, JE
通讯作者：
DOWLING, JE