Metabolomic profiling of stool of two-year old children from the INSIGHT study reveals links between butyrate and child weight outcomes.
Metabolomic profiling of stool of two-year old children from the INSIGHT study reveals links between butyrate and child weight outcomes.
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来自洞察力研究的两岁儿童凳子的代谢组分析揭示了丁酸酯与儿童体重结局之间的联系。
DOI：
10.1111/ijpo.12833
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发表时间：
2022-01
影响因子：
3.8
通讯作者：
Chiaromonte F
中科院分区：
文献类型：
--
作者：
Nandy D;Craig SJC;Cai J;Tian Y;Paul IM;Savage JS;Marini ME;Hohman EE;Reimherr ML;Patterson AD;Makova KD;Chiaromonte F
代谢组学分析通常用于了解肥胖等疾病的生物学基础。然而，我们对肠道代谢物与幼儿体重结果相关的知识目前有限。(1)探索代谢物与儿童体重结局之间的关系；(2)确定协变量（如儿童饮食、孕期孕产妇健康/习惯等）在代谢物与儿童体重结局之间的潜在影响；(3)探索所选肠道代谢物与肠道微生物群丰度之间的关系。利用1H - NMR，我们从170名两岁儿童的粪便样本中定量测定了30种代谢物。为了确定与儿童体重结局相关的代谢物和协变量（BMI[体重/身高2]、BMI z评分[年龄和性别调整后的BMI]和生长指数[体重/身高]），我们使用LASSO和最佳子集选择回归技术分析了1H‐NMR数据以及记录在儿童和母亲上的20个协变量。来自相同粪便样本的先前表征的微生物群落信息用于确定选定的肠道代谢物和肠道微生物群之间的关联。2岁时，粪便丁酸盐浓度与儿童BMI （p值= 3.58 × 10-4）、BMI z评分（p值= 3.47 × 10-4）和生长指数（p值= 7.73 × 10-4）呈显著正相关。协变量，如孕妇在怀孕期间吸烟是需要考虑的重要因素。丁酸盐浓度与细菌属Faecalibacterium的丰度呈正相关（p‐value = 9.61 × 10−3）。粪便丁酸盐浓度与儿童体重增加呈正相关，应进一步研究其作为影响儿童肥胖的因素。
Metabolomic analysis is commonly used to understand the biological underpinning of diseases such as obesity. However, our knowledge of gut metabolites related to weight outcomes in young children is currently limited. To (1) explore the relationships between metabolites and child weight outcomes, (2) determine the potential effect of covariates (e.g., child's diet, maternal health/habits during pregnancy, etc.) in the relationship between metabolites and child weight outcomes, and (3) explore the relationship between selected gut metabolites and gut microbiota abundance. Using 1H‐NMR, we quantified 30 metabolites from stool samples of 170 two‐year‐old children. To identify metabolites and covariates associated with children's weight outcomes (BMI [weight/height2], BMI z‐score [BMI adjusted for age and sex], and growth index [weight/height]), we analysed the 1H‐NMR data, along with 20 covariates recorded on children and mothers, using LASSO and best subset selection regression techniques. Previously characterized microbiota community information from the same stool samples was used to determine associations between selected gut metabolites and gut microbiota. At age 2 years, stool butyrate concentration had a significant positive association with child BMI (p‐value = 3.58 × 10–4), BMI z‐score (p‐value = 3.47 × 10–4), and growth index (p‐value = 7.73 × 10–4). Covariates such as maternal smoking during pregnancy are important to consider. Butyrate concentration was positively associated with the abundance of the bacterial genus Faecalibacterium (p‐value = 9.61 × 10−3). Stool butyrate concentration is positively associated with increased child weight outcomes and should be investigated further as a factor affecting childhood obesity.
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影响因子：
6.4
作者：
Benitez-Paez, Alfonso;Kjlbaek, Louise;Sanz, Yolanda
通讯作者：
Sanz, Yolanda
毒理学家的肠道微生物组的结构和功能分析。
DOI：
10.1002/cptx.54
发表时间：
2018-11
期刊：
Current protocols in toxicology
影响因子：
--
作者：
Nichols RG;Cai J;Murray IA;Koo I;Smith PB;Perdew GH;Patterson AD
通讯作者：
Patterson AD
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
4.6
作者：
Craig, Sarah J. C.;Blankenberg, Daniel;Makova, Kateryna D.
通讯作者：
Makova, Kateryna D.
DOI：
10.2307/2348411
发表时间：
1996-01-01
期刊：
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES D-THE STATISTICIAN
影响因子：
--
作者：
Gilmour, SG
通讯作者：
Gilmour, SG