In-situ ultra-sensitive infrared absorption spectroscopy of biomolecule interactions in real time with plasmonic nanoantennas.
In-situ ultra-sensitive infrared absorption spectroscopy of biomolecule interactions in real time with plasmonic nanoantennas.
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DOI：
10.1038/ncomms3154
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发表时间：
2013
影响因子：
16.6
通讯作者：
Altug, Hatice
中科院分区：
文献类型：
--
作者：
Adato, Ronen;Altug, Hatice
红外吸收光谱是一种强大的生化分析工具，因为它以无标记的方式提取详细的分子结构信息。其分子特异性使得该技术对蛋白质响应于各种刺激而表现出的微妙构象变化敏感。然而，灵敏度限制和液态水的极强吸收带严重限制了红外光谱法在生物分子的天然水性环境中进行动力学测量。在这里，我们展示了一种基于等离子体芯片的技术，该技术克服了这些挑战，能够在真实的时间内以高灵敏度原位监测蛋白质和纳米颗粒的相互作用，甚至允许在结合事件期间观察微小体积的水位移。我们的方法利用了吸收带的等离子体增强以及非经典形式的内反射。这些功能不仅将红外光谱的范围扩展到一类新的生物相互作用，而且还实现了独特的基于芯片的技术。 红外吸收光谱提供了关于分子的重要信息，但受到水吸收的阻碍。Adato和Altug利用纳米天线的等离子体增强来克服这一点，从而实现对水环境中生物样品的基于芯片的监测。
Infrared absorption spectroscopy is a powerful biochemical analysis tool as it extracts detailed molecular structural information in a label-free fashion. Its molecular specificity renders the technique sensitive to the subtle conformational changes exhibited by proteins in response to a variety of stimuli. Yet, sensitivity limitations and the extremely strong absorption bands of liquid water severely limit infrared spectroscopy in performing kinetic measurements in biomolecules’ native, aqueous environments. Here we demonstrate a plasmonic chip-based technology that overcomes these challenges, enabling the in-situ monitoring of protein and nanoparticle interactions at high sensitivity in real time, even allowing the observation of minute volumes of water displacement during binding events. Our approach leverages the plasmonic enhancement of absorption bands in conjunction with a non-classical form of internal reflection. These features not only expand the reach of infrared spectroscopy to a new class of biological interactions but also additionally enable a unique chip-based technology. Infrared absorption spectroscopy provides important information about molecules, but is hampered by the absorption of water. Adato and Altug exploit the plasmonic enhancement from nanoantennas to overcome this, enabling chip-based monitoring of biological samples in aqueous environments.
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DOI：
10.1073/pnas.0907459106
发表时间：
2009-11-17
影响因子：
11.1
作者：
Adato, Ronen;Yanik, Ahmet A.;Altug, Hatice
通讯作者：
Altug, Hatice
影响因子：
10.8
作者：
Adato, Ronen;Yanik, Ahmet A.;Altug, Hatice
通讯作者：
Altug, Hatice
影响因子：
3.3
作者：
Badura, Adrian;Esper, Berndt;Roegner, Matthias
通讯作者：
Roegner, Matthias
影响因子：
15
作者：
Ataka, K;Heberle, J
通讯作者：
Heberle, J
影响因子：
10.8
作者：
Artar, Alp;Yanik, Ahmet A.;Altug, Hatice
通讯作者：
Altug, Hatice