Mechanisms for intracellular distribution of mRNA: in situ hybridization studies in muscle.
Mechanisms for intracellular distribution of mRNA: in situ hybridization studies in muscle.
复制标题
mRNA 细胞内分布的机制：肌肉中的原位杂交研究。
DOI：
10.1152/ajpcell.1992.262.1.c1
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Dix,DJ
中科院分区：
文献类型：
--
作者：
Russell,B;Dix,DJ
横纹肌纤维中mRNA的细胞内分布是高度有序的，纤维收缩器的结构组织也是如此。回顾肌肉mRNA原位杂交的结果，试图辨别mRNA分布所涉及的机制，并确定其与肌肉发育、生长和修复过程的关系。非放射性标记的互补RNA探针允许在光镜和电镜水平上对mRNA进行解剖定位。横纹肌中的肌球蛋白mRNA集中在转录活性核周围，肌球蛋白mRNA被肌原纤维质量排除在外，肌球蛋白mRNA分布与细胞骨架元素相关，肌球蛋白mRNA集中在快速生长和修复的区域。肌球蛋白mRNA沿着肌原纤维长度的均匀分布并不表明与粗肌丝或细肌丝的特异性关联。在指导mRNA分布的可能机制中，原位杂交和其他分析的结果支持限制扩散模型。mRNA（和多聚核糖体）的扩散受到肌原纤维晶格的严重限制。肌球蛋白mRNA也可能与细胞骨架元件相关，这可能将mRNA引导到特定的细胞内位置并影响翻译活性。
The intracellular distribution of mRNA in striated muscle fibers is highly ordered, as is the structural organization of the fibers' contractile apparatus. Results from in situ hybridization of muscle mRNA are reviewed in an attempt to discern the mechanisms involved in mRNA distribution and to determine its relationship to developmental, growth, and repair processes in muscle. Nonradioactively labeled complementary RNA probes allow anatomic localization of mRNA at the light and electron microscopic level. Myosin mRNA in striated muscle is concentrated around transcriptionally active nuclei, myosin mRNA is excluded by the myofibrillar mass, myosin mRNA distribution correlates with that of cytoskeletal elements, and myosin mRNA is concentrated in regions of rapid growth and repair. The even distribution of myosin mRNA along the length of myofibrils gives no indication of specific association with either the thick or thin filaments. Of the possible mechanisms directing mRNA distribution, results from in situ hybridization and other analyses support a restricted diffusion model. Diffusion of mRNA (and polysomes) is severely limited by the myofibrillar lattice. It is possible that myosin mRNA is also associated with a cytoskeletal element, which may direct the mRNA to specific intracellular locations and affect translational activity.
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影响因子：
64.8
作者：
P. Macdonald;G. Struhl
通讯作者：
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影响因子：
64.5
作者：
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影响因子：
5.2
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1177/39.8.1856452
发表时间：
1991-08
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
M. Wenderoth;B. Eisenberg
通讯作者：
M. Wenderoth;B. Eisenberg