Genistein induces increase in fluid pH, Na+ and HCO3(-) concentration, SLC26A6 and SLC4A4 (NBCe1)-B expression in the uteri of ovariectomized rats.
Genistein induces increase in fluid pH, Na+ and HCO3(-) concentration, SLC26A6 and SLC4A4 (NBCe1)-B expression in the uteri of ovariectomized rats.
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DOI：
10.3390/ijms15010958
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发表时间：
2014-01-10
影响因子：
5.6
通讯作者：
Salleh N
中科院分区：
文献类型：
--
作者：
Chinigarzadeh A;Kasim NF;Muniandy S;Kassim NM;Salleh N
据报道，染料木黄酮可刺激管腔HCO 3-分泌。我们假设染料木素通过SLC 26 A6和SLC 4A 4（NBCe 1）转运蛋白介导这种作用。我们的研究旨在：研究金雀异黄素作用下子宫液pH、Na+和HCO 3 −浓度以及子宫SLC 26 A6和NBCe 1表达的变化。切除卵巢的成年雌性大鼠接受25、50和100 mg/kg/天的染料木黄酮，持续一周，伴或不伴ICI 182780。最后一次注射后一天，进行体内子宫灌注以收集子宫液用于Na+、HCO 3 −和pH值测定。然后处死动物，取出子宫进行mRNA和蛋白质表达分析。通过免疫组织化学（IHC）观察SLC 26 A6和NBCe 1-A和NBCe 1-B分布。50和100 mg/kg/天的染料木黄酮刺激子宫液pH值、Na+和HCO 3 −浓度升高。100 mg/kg/天的染料木黄酮上调SLC 26 A6和SLC 4A 4 mRNA的表达，其在伴随ICI 182780给药后降低。与此同时，SLC 26 A6和NBCe 1-B蛋白表达也在高剂量金雀异黄素处理后增加，并且主要定位于腔上皮的顶膜。染料木黄酮上调SLC 26 A6和NBCe 1-B可能是在这种条件下观察到的子宫液pH值、Na+和HCO 3 −浓度增加的原因。
Genistein has been reported to stimulate luminal HCO3− secretion. We hypothesized that genistein mediates this effect via SLC26A6 and SLC4A4 (NBCe1) transporters. Our study aimed to: investigate changes in uterine fluid pH, Na+ and HCO3− concentration and expression of uterine SLC26A6 and NBCe1 under genistein effect. Ovariectomized adult female rats received 25, 50 and 100 mg/kg/day genistein for a week with and without ICI 182780. A day after the last injection, in vivo uterine perfusion was performed to collect uterine fluid for Na+, HCO3− and pH determination. The animals were then sacrificed and uteri were removed for mRNA and protein expression analyses. SLC26A6 and NBCe1-A and NBCe1-B distribution were visualized by immunohistochemistry (IHC). Genistein at 50 and 100 mg/kg/day stimulates uterine fluid pH, Na+ and HCO3− concentration increase. Genistein at 100 mg/kg/day up-regulates the expression of SLC26A6 and SLC4A4 mRNA, which were reduced following concomitant ICI 182780 administration. In parallel, SLC26A6 and NBCe1-B protein expression were also increased following high dose genistein treatment and were localized mainly at the apical membrane of the luminal epithelia. SLC26A6 and NBCe1-B up-regulation by genistein could be responsible for the observed increase in the uterine fluid pH, Na+ and HCO3− concentration under this condition.
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影响因子：
3.2
作者：
Kurtz I;Zhu Q
通讯作者：
Zhu Q
影响因子：
5.5
作者：
Lee, Seong-Ki;Boron, Walter F.;Parker, Mark D.
通讯作者：
Parker, Mark D.
DOI：
10.1007/s00360-011-0622-y
发表时间：
2012-04-01
影响因子：
2
作者：
Bucking, Carol;Wood, Chris M.
通讯作者：
Wood, Chris M.
影响因子：
4.4
作者：
Lohi, H;Kujala, M;Kere, J
通讯作者：
Kere, J
影响因子：
1.1
作者：
Little, DL
通讯作者：
Little, DL