RP-HPLC characterization of lupenone and β-sitosterol in rhizoma musae and evaluation of the anti-diabetic activity of lupenone in diabetic Sprague-Dawley rats.
RP-HPLC characterization of lupenone and β-sitosterol in rhizoma musae and evaluation of the anti-diabetic activity of lupenone in diabetic Sprague-Dawley rats.
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芭蕉中羽扇豆酮和 β-谷甾醇的 RP-HPLC 表征以及羽扇豆酮在糖尿病 Sprague-Dawley 大鼠中的抗糖尿病活性评价
DOI：
10.3390/molecules190914114
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发表时间：
2014-09-09
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Xu F;Wu H;Wang X;Yang Y;Wang Y;Qian H;Zhang Y
为了鉴别番荔枝药材中的有效成分羽扇豆烯酮和β-谷甾醇，建立了分离番荔枝药材中这两种有效成分的反相高效液相色谱方法(回归系数为0.9996)。并将该方法应用于不同生长环境下栽培的番荔枝样品中羽扇豆烯酮和β-谷甾醇的含量测定。不同的地理位置、生育期和采收期等变量对羽扇豆酮和β-谷甾醇水平有不同的影响。此外，我们还确定了番荔枝药材栽培和采收的最佳条件。羽扇豆酮1.78、5.33、16.0 0 mg·kg-1·d-−-1连续给药14d可显著降低糖尿病大鼠的空腹血糖(Fbg)，糖化血红蛋白(Hb-A1c)在5.33、16.0 0 mg·kg-1·d-−-1剂量下也显著降低，显示出较强的抗糖尿病活性。据我们所知，这是首次报道优化的高效液相色谱法成功地应用于羽扇豆烯酮和β-谷甾醇的定量，并将其应用于番荔枝生长的优化。动物实验也首次表明番荔枝根中的羽扇豆烯酮具有抗糖尿病活性。
With the aim of characterizing the active ingredients lupenone and β-sitosterol in Rhizoma Musae samples a reversed-phase HPLC method for the separation of these two compounds in Rhizoma Musae samples was developed (regression coefficient > 0.9996). The method was further applied to quantify lupenone and β-sitosterol content in Rhizoma Musae samples cultured in different growth environments. Different variables such as geographical location, growth stage, and harvest time, demonstrated differential effects on lupenone and β-sitosterol levels. Moreover, we determined the optimum conditions for cultivation and harvesting of Rhizoma Musae herbs. Lupenone administration caused a significant reduction in fasting blood glucose (FBG) levels in diabetic rats at doses of 1.78, 5.33, and 16.00 mg·kg−1·day−1 for 14 days, the glycated hemoglobin (HbA1c) levels of diabetic rats also significantly reduced at doses of 5.33, and 16.00 mg·kg−1·day−1, indicating a robust antidiabetic activity. To our knowledge, this is the first report of an optimized HPLC method successfully applied to quantify lupenone and β-sitosterol, and its applicability in optimizing Rhizoma Musae growth. Animal experiments also showed for the first time that lupenone from Rhizoma Musae has anti-diabetic activity.
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DOI：
10.1080/14756360802693312
发表时间：
2009-08-01
影响因子：
5.6
作者：
Na, Minkyun;Kim, Bo Yeon;Ahn, Jong Seog
通讯作者：
Ahn, Jong Seog
影响因子：
2.7
作者：
Villareal, Myra O.;Han, Junkyu;Isoda, Hiroko
通讯作者：
Isoda, Hiroko
影响因子：
2.9
作者：
von Holtz, RL;Fink, CS;Awad, AB
通讯作者：
Awad, AB
影响因子：
6.1
作者：
Darji K;Miglis C;Wardlow A;Abourashed EA
通讯作者：
Abourashed EA
影响因子：
--
作者：
Baskar, Albert A.;Ignacimuthu, Savarimuthu;Al Numair, Khalid S.
通讯作者：
Al Numair, Khalid S.