Evaluation of Circulating Endometrial Cells as a Biomarker for Endometriosis.
Evaluation of Circulating Endometrial Cells as a Biomarker for Endometriosis.
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循环子宫内膜细胞作为子宫内膜异位症生物标志物的评估
DOI：
10.4103/0366-6999.215325
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发表时间：
2017-10-05
影响因子：
6.1
通讯作者：
Chang XH
中科院分区：
文献类型：
--
作者：
Chen Y;Zhu HL;Tang ZW;Neoh KH;Ouyang DF;Cui H;Cheng HY;Ma RQ;Ye X;Han RP;Chang XH
背景：据报道，循环子宫内膜细胞（CECs）存在于子宫内膜异位症（EM）女性的外周血中，为异位病变的存在提供了明确和特异性的证据。本研究建立了一种高检出率的CECs检测方法，评估CECs对EM的诊断价值，并与血清CA125进行比较，从CECs的新视角对EM的发病机制提出假设。方法：于2015年10月至2016年7月进行前瞻性研究。采集59例受试者外周血，分离血细胞，利用微流控芯片进行免疫荧光染色。波形蛋白/细胞角蛋白和雌激素/孕酮受体阳性，CD45阴性的细胞为CECs。采用电化学发光免疫分析法检测血清CA125水平。结果：EM组CECs检出率为89.5%(17/19)，显著高于对照组（15.0% [6/40],P < 0.001），且与月经周期无关。此外，CEC阳性检测检测出4/5的I-II期EM。相比之下，CA125阳性检测在检测EM方面的价值有限（13/19,68.4%），仅检测出1例I-II期EM。结论：CEC是有希望的EM生物标志物，具有巨大的无创诊断检测潜力。
Background: Circulating endometrial cells (CECs) have been reported to be present in the peripheral blood of women with endometriosis (EM), providing clear and specific evidence of the presence of ectopic lesions. In this study, we established a method with a high detection rate of CECs, assessed the diagnostic value of CECs for EM and compared with serum CA125, and proposed a hypothesis for the pathogenesis of EM from the new perspective of CECs. Methods: The participants were enrolled prospectively from October 2015 to July 2016. The peripheral blood samples were collected from 59 participants, and the blood cells were isolated for immunofluorescence staining via microfluidic chips. The cells that were positive for vimentin/cytokeratin and estrogen/progesterone receptor and negative for CD45 were identified as CECs. The serum CA125 level was tested with electrochemiluminescence immunoassay. Results: The detection rate of CECs reached 89.5% (17/19) in the EM group, which was significantly higher than that of the control group (15.0% [6/40], P < 0.001) and was independent of menstrual cycle phases. Furthermore, a positive CEC assay detected 4/5 cases of Stage I–II EM. In contrast, a positive CA125 test had limited value in detecting EM (13/19, 68.4%) and detected only one case of Stage I–II EM. Conclusion: CECs are promising biomarkers for EM with great potential for a noninvasive diagnostic assay.
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影响因子：
6.7
作者：
Louis, Germaine M. Buck;Hediger, Mary L.;Peterson, C. Matthew;Croughan, Mary;Sundaram, Rajeshwari;Stanford, Joseph;Chen, Zhen;Fujimoto, Victor Y.;Varner, Michael W.;Trumble, Ann;Giudice, Linda C.
通讯作者：
Giudice, Linda C.
DOI：
10.1016/j.bbamcr.2015.09.002
发表时间：
2015-11-01
影响因子：
5.1
作者：
Saavedra, Paula;Girona, Josefa;Masana, Lluis
通讯作者：
Masana, Lluis
影响因子：
--
作者：
Fassbender A;Burney RO;O DF;D'Hooghe T;Giudice L
通讯作者：
Giudice L
影响因子：
2.9
作者：
Wang, Lei;Huang, Wei;Xia, Xiaomeng
通讯作者：
Xia, Xiaomeng
激素受体表达和卵巢癌存活：卵巢肿瘤组织分析联盟研究。
DOI：
10.1016/s1470-2045(13)70253-5
发表时间：
2013-08
期刊：
The Lancet. Oncology
影响因子：
--
作者：
Sieh W;Köbel M;Longacre TA;Bowtell DD;deFazio A;Goodman MT;Høgdall E;Deen S;Wentzensen N;Moysich KB;Brenton JD;Clarke BA;Menon U;Gilks CB;Kim A;Madore J;Fereday S;George J;Galletta L;Lurie G;Wilkens LR;Carney ME;Thompson PJ;Matsuno RK;Kjær SK;Jensen A;Høgdall C;Kalli KR;Fridley BL;Keeney GL;Vierkant RA;Cunningham JM;Brinton LA;Yang HP;Sherman ME;García-Closas M;Lissowska J;Odunsi K;Morrison C;Lele S;Bshara W;Sucheston L;Jimenez-Linan M;Driver K;Alsop J;Mack M;McGuire V;Rothstein JH;Rosen BP;Bernardini MQ;Mackay H;Oza A;Wozniak EL;Benjamin E;Gentry-Maharaj A;Gayther SA;Tinker AV;Prentice LM;Chow C;Anglesio MS;Johnatty SE;Chenevix-Trench G;Whittemore AS;Pharoah PD;Goode EL;Huntsman DG;Ramus SJ
通讯作者：
Ramus SJ