Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology.
Chilling injury of tomato fruit was alleviated under low-temperature storage by silencing Sly-miR171e with short tandem target mimic technology.
复制标题
短串联靶模拟技术沉默Sly-miR171e减轻番茄果实低温贮藏冷害
DOI：
10.3389/fnut.2022.906227
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发表时间：
2022
影响因子：
5
通讯作者：
Xu, Xiangbin
中科院分区：
文献类型：
--
作者：
Zhao, Keyan;Chen, Rulong;Duan, Wenhui;Meng, Lanhuan;Song, Hongmiao;Wang, Qing;Li, Jiangkuo;Xu, Xiangbin
本研究研究了Sly-miR171e对番茄果实采后耐寒性的影响。结果表明，Sly-miR171e（miR171e-OE）的过表达促进番茄果实红成熟（MR）和绿成熟（MG）阶段的采后冷害（CI）。与野生型（WT）和miR171e-OE果实相比，Sly-miR171e（miR171e-STTM）的敲低表现出较低的CI指数、较低的过氧化氢（H2O2）含量和较高的采收后果实硬度。 miR171e-STTM果实中GRAS24、CBF1、GA2ox1、COR的表达量和GA3含量上升，GA20ox1、GA3ox1的表达量下降。研究表明，通过短串联靶点模拟（STTM）技术沉默 Sly-miR171e，可以减轻低温储存时番茄果实的 CI。此外，它还为miR171e的遗传修饰和采后果实CI的控制提供了有用的信息。
In this study, the role of Sly-miR171e on post-harvest cold tolerance of tomato fruit was researched. The results showed that overexpression of Sly-miR171e (miR171e-OE) promoted postharvest chilling injury (CI) of tomato fruit at the mature red (MR) and mature green (MG) stage. Contrasted with the wild type (WT) and miR171e-OE fruit, the knockdown of Sly-miR171e (miR171e-STTM) showed a lower CI index, lower hydrogen peroxide (H2O2) content, and higher fruit firmness after harvest. In the fruit of miR171e-STTM, the expression level of GRAS24, CBF1, GA2ox1, and COR, and the GA3 content were ascended, while the expression levels of GA20ox1 and GA3ox1 were descended. The research demonstrated that CI in tomato fruit was alleviated at low temperature storage by silencing Sly-miR171e with short tandem target mimic (STTM) technology. Furthermore, it also provided helpful information for genetic modification of miR171e and control of CI in the postharvest fruit.
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