Xanthomonas adaptation to common bean is associated with horizontal transfers of genes encoding TAL effectors.
Xanthomonas adaptation to common bean is associated with horizontal transfers of genes encoding TAL effectors.
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Xanthomonas适应共同豆类与编码TAL效应子的基因的水平转移有关。
DOI：
10.1186/s12864-017-4087-6
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发表时间：
2017-08-30
期刊：
影响因子：
4.4
通讯作者：
Chen NWG
中科院分区：
文献类型：
--
作者：
Ruh M;Briand M;Bonneau S;Jacques MA;Chen NWG
菜豆白叶枯病是由柑桔黄单胞菌致病变种引起的菜豆细菌性病害。镰刀菌和菜豆黄单胞菌致病变种（Xanthomonashaseoli pv.）菜豆这些远缘菌株能够在菜豆上引起相似的症状，表明它们已经获得了适应菜豆的共同遗传决定因素。转录激活因子样（TAL）效应物是细菌III型效应物，能够诱导宿主基因的表达以促进感染或抗性。它们与特定宿主DNA序列结合的能力表明它们是宿主适应的潜在候选者。为了研究菜豆白叶枯病病原菌黄单胞菌tal基因的多样性，对代表菜豆白叶枯病病原菌多样性的17株黄单胞菌进行了全基因组测序。Citri PV. fuscans和X.菜豆变种通过单分子真实的时间测序获得菜豆。对这些基因组的分析表明存在四个tal基因，分别命名为tal 23 A、tal 20 F、tal 18 G和tal 18 H。tal 20 F和tal 18 G是染色体组的，而tal 23 A和tal 18 H则携带在质粒上，并在遗传学上相距较远的菌株之间共享，因此表明这些基因最近在X. Citri PV. fuscans和X.菜豆变种菜豆菌株。值得注意的是，tal 23 A存在于所研究的所有菌株中，这表明它在适应普通菜豆中起重要作用。通过计算机模拟对TAL效应子在菜豆基因组中的作用靶点进行预测，结果表明TAL效应子与X。Citri PV. fuscans和X.菜豆变种菜豆菌株靶向具有相似功能的基因的启动子。这可能是来自不同系统发育组的TAL效应子之间趋同进化的痕迹，并安慰TAL效应子已被暗示在对菜豆的适应中的假设。总而言之，我们的研究结果有利于一个模型，其中质粒TAL效应子能够通过在遥远的谱系之间水平转移来促进宿主适应。本文的在线版本（10.1186/s12864-017-4087-6）包含补充材料，可供授权用户使用。
Common bacterial blight is a devastating bacterial disease of common bean (Phaseolus vulgaris) caused by Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli. These phylogenetically distant strains are able to cause similar symptoms on common bean, suggesting that they have acquired common genetic determinants of adaptation to common bean. Transcription Activator-Like (TAL) effectors are bacterial type III effectors that are able to induce the expression of host genes to promote infection or resistance. Their capacity to bind to a specific host DNA sequence suggests that they are potential candidates for host adaption. To study the diversity of tal genes from Xanthomonas strains responsible for common bacterial blight of bean, whole genome sequences of 17 strains representing the diversity of X. citri pv. fuscans and X. phaseoli pv. phaseoli were obtained by single molecule real time sequencing. Analysis of these genomes revealed the existence of four tal genes named tal23A, tal20F, tal18G and tal18H, respectively. While tal20F and tal18G were chromosomic, tal23A and tal18H were carried on plasmids and shared between phylogenetically distant strains, therefore suggesting recent horizontal transfers of these genes between X. citri pv. fuscans and X. phaseoli pv. phaseoli strains. Strikingly, tal23A was present in all strains studied, suggesting that it played an important role in adaptation to common bean. In silico predictions of TAL effectors targets in the common bean genome suggested that TAL effectors shared by X. citri pv. fuscans and X. phaseoli pv. phaseoli strains target the promoters of genes of similar functions. This could be a trace of convergent evolution among TAL effectors from different phylogenetic groups, and comforts the hypothesis that TAL effectors have been implied in the adaptation to common bean. Altogether, our results favour a model where plasmidic TAL effectors are able to contribute to host adaptation by being horizontally transferred between distant lineages. The online version of this article (10.1186/s12864-017-4087-6) contains supplementary material, which is available to authorized users.
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影响因子：
13.8
作者：
Blanvillain-Baufumé S;Reschke M;Solé M;Auguy F;Doucoure H;Szurek B;Meynard D;Portefaix M;Cunnac S;Guiderdoni E;Boch J;Koebnik R
通讯作者：
Koebnik R
DOI：
10.1128/genomea.00032-12
发表时间：
2013-01
期刊：
Genome announcements
影响因子：
--
作者：
Bolot S;Guy E;Carrere S;Barbe V;Arlat M;Noël LD
通讯作者：
Noël LD
影响因子：
56.9
作者：
Boch, Jens;Scholze, Heidi;Bonas, Ulla
通讯作者：
Bonas, Ulla
影响因子：
3.3
作者：
Baker, Richard;Bragard, Claude;van der Werf, Wopke
通讯作者：
van der Werf, Wopke
Xanthomonas fuscans亚种的基因组序列。 Fuscans菌株4834-R揭示了鞭毛运动并不是黄褐色的一般特征。
影响因子：
4.4
作者：
Darrasse A;Carrère S;Barbe V;Boureau T;Arrieta-Ortiz ML;Bonneau S;Briand M;Brin C;Cociancich S;Durand K;Fouteau S;Gagnevin L;Guérin F;Guy E;Indiana A;Koebnik R;Lauber E;Munoz A;Noël LD;Pieretti I;Poussier S;Pruvost O;Robène-Soustrade I;Rott P;Royer M;Serres-Giardi L;Szurek B;van Sluys MA;Verdier V;Vernière C;Arlat M;Manceau C;Jacques MA
通讯作者：
Jacques MA