The diatom-derived aldehyde decadienal affects life cycle transition in the ascidian Ciona intestinalis through nitric oxide/ERK signalling.
The diatom-derived aldehyde decadienal affects life cycle transition in the ascidian Ciona intestinalis through nitric oxide/ERK signalling.
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DOI：
10.1098/rsob.140182
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发表时间：
2015-03
期刊：
影响因子：
5.8
通讯作者：
Palumbo A
中科院分区：
文献类型：
--
作者：
Castellano I;Ercolesi E;Romano G;Ianora A;Palumbo A
多不饱和醛 (PUA) 是一些微藻（包括不同的硅藻物种）产生的脂肪酸衍生代谢物。 PUA 主要是作为针对微藻捕食者的伤口激活防御机制而产生的，或者是在花期结束时从衰老细胞中释放出来的。 PUA，包括 2,4-反式十年醛 (DD)，会对多种浮游和底栖生物的胚胎和幼虫发育产生有害影响。在这里，我们报告了 DD 对海鞘幼虫发育和变态的影响。海鞘幼虫的发育受到不同分子事件的相互作用的调节，包括一氧化氮 (NO) 的产生、ERK 的激活和 caspase 3 依赖性细胞凋亡。我们报告说，在有能力的幼虫阶段用 DD 进行治疗会导致变态延迟。 DD 通过降低总谷胱甘肽和一氧化氮水平来影响氧化还原平衡。通过生化和定量基因表达分析，我们确定了受 DD 影响的 NO 信号传导网络，包括 ERK 磷酸酶 mkp1 的上调和随之而来的 ERK 磷酸化的减少，以及下游 ERK 靶基因表达的最终变化。总体而言，这些结果为海洋生物在幼体发育过程中接触 PUA 后诱导的分子途径提供了新的见解，表明这种醛影响幼体从营养生命阶段过渡到生殖生命阶段的关键检查点。
Polyunsaturated aldehydes (PUAs) are fatty-acid-derived metabolites produced by some microalgae, including different diatom species. PUAs are mainly produced as a wound-activated defence mechanism against microalgal predators or released from senescent cells at the end of a bloom. PUAs, including 2,4-trans-decadienal (DD), induce deleterious effects on embryonic and larval development of several planktonic and benthic organisms. Here, we report on the effects of DD on larval development and metamorphosis of the ascidian Ciona intestinalis. Ciona larval development is regulated by the cross-talking of different molecular events, including nitric oxide (NO) production, ERK activation and caspase 3-dependent apoptosis. We report that treatment with DD at the competence larval stage results in a delay in metamorphosis. DD affects redox balance by reducing total glutathione and NO levels. By biochemical and quantitative gene expression analysis, we identify the NO-signalling network affected by DD, including the upregulation of ERK phosphatase mkp1 and consequent reduction of ERK phosphorylation, with final changes in the expression of downstream ERK target genes. Overall, these results give new insights into the molecular pathways induced in marine organisms after exposure to PUAs during larval development, demonstrating that this aldehyde affects key checkpoints of larval transition from the vegetative to the reproductive life stage.
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影响因子：
4.8
作者：
Cassano, Silvana;Agnese, Savina;Avvedimento, Enrico Vittorio
通讯作者：
Avvedimento, Enrico Vittorio
影响因子：
1.8
作者：
Fontana, Angolo;d'Ippolito, Giuliana;Cimino, Guido
通讯作者：
Cimino, Guido
影响因子：
4.5
作者：
Franchi, N.;Ferro, D.;Santovito, G.
通讯作者：
Santovito, G.
影响因子：
3.2
作者：
Fontana, Angelo;d'Ippolito, Giuliana;Ianora, Adrianna
通讯作者：
Ianora, Adrianna
影响因子：
3.7
作者：
Castellano I;Ercolesi E;Palumbo A
通讯作者：
Palumbo A