Label-free surface-enhanced Raman spectroscopy detection of absorption manner of lysozyme based on nanodots arrays
Label-free surface-enhanced Raman spectroscopy detection of absorption manner of lysozyme based on nanodots arrays
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基于纳米点阵列的无标记表面增强拉曼光谱检测溶菌酶吸收方式
DOI：
10.1016/j.apsusc.2020.145332
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发表时间：
2020-04
影响因子：
6.7
通讯作者：
Geng Yanquan
中科院分区：
文献类型：
--
作者：
Wang Jiqiang;Yan Yongda;Chang Shunyu;Han Yimin;Geng Yanquan
溶菌酶在动物或人体的先天免疫系统中发挥着重要的作用，因此，采用一种高灵敏度和高效率的方法检测溶菌酶在临床中至关重要。基于纳米结构的表面增强拉曼散射（SERS）衬底经常被用于检测生物分子。在本研究中，利用afm探针采用二次扫描刮擦策略制备了纳米点阵列结构作为微纳混合SERS衬底。研究了纳米点结构与法向载荷和划痕角度等加工参数的关系。研究了纳米结构特征尺寸和金膜厚度对罗丹明6G (R6G) SERS光谱的影响。此外，利用不同干燥温度和浓度的nano3溶液，研究了溶菌酶在Ag底物上的吸附方式。研究了溶菌酶和胰岛素二元蛋白混合物的SERS光谱。结果表明，利用AFM纳米加工方法制备的基底可以促进SERS在生物领域的应用。
Lysozyme plays an important role in the innate immune system of animal or human body, thus, it is vital to detect it using a high sensitivity and efficiency approach in clinic. Nanostructure-based Surface-enhanced Raman scattering (SERS) substrate is often employed to detect biomolecules. In this study, a nanodots array structure, which was utilized as a micro-nano hybrid SERS substrate, was fabricated using second-scan scratching strategy with AFM-probe. The dependence of the nanodots structure on the machining parameters such as applied normal loads and scratching angles were investigated. The effects of the nanostructure feature sizes and the Au film thickness on the measured SERS spectra that tested using Rhodamine 6G (R6G) were studied. In addition, different drying temperatures and concentrations of NaNO3solution were used to study the absorption manner of lysozyme on an Ag substrate. The SERS spectra of a binary protein mixture, which includes lysozyme and insulin, were also investigated based on the fabricated nanostructures. Results showed that the substrates fabricated using AFM nanomachining approach may promote the SERS application in biologic field.
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影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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