Ontogeny of receptors for insulin-like peptides in chick embryo tissues: early dominance of insulin-like growth factor over insulin receptors in brain.
Ontogeny of receptors for insulin-like peptides in chick embryo tissues: early dominance of insulin-like growth factor over insulin receptors in brain.
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鸡胚组织中胰岛素样肽受体的个体发育：胰岛素样生长因子在大脑中对胰岛素受体的早期优势。
作者：
L. Bassas;F. Pablo;M. Lesniak;J. Roth
最近，我们证实了早期数据显示外源性胰岛素对鸡胚胎发育第2天的有害影响，尽管胰岛素受体直到第3-4天才被清楚地证明。现在我们报道胰岛素样生长因子（IGF）受体在第2天就出现在整个胚胎中。[125I]IGF-I和[125I]IGF-II与脑结合的发育模式相似，IGF-I的结合率约为IGF-II的2倍；从第3天到第6天急剧增加，随后在个体发生的第二和第三周逐渐下降。与未标记类似物的竞争性结合实验表明，两种标记的IGF都与I型IGF受体结合，胰岛素与它们相互作用。与其他已知的IGF受体相比，其对温度和pH的依赖性相对较高。我们之前报道过，[125I]胰岛素与大脑的结合在第3天几乎检测不到，并在胚胎生命的其余时间里逐渐增加。IGF和胰岛素受体的模式似乎是器官特异性的，因为心脏、肝脏和肢体芽等非神经组织在个体发生中表现出不同的结合特征。由此可见，IGF受体在鸡胚脑中发育早于胰岛素受体，并可能在胚胎发生早期介导IGF和胰岛素的作用。
Recently, we confirmed early data showing deleterious effects of exogenous insulin on chick embryos at 2 days of development, although insulin receptors were not clearly demonstrable until days 3-4. Now we report that insulin-like growth factor (IGF) receptors are present in whole embryos on day 2. The developmental patterns of [125I]IGF-I and [125I]IGF-II binding to brain were similar, and IGF-I showed approximately a 2-fold higher binding than IGF-II; there was a sharp increase from days 3 to 6, and a subsequent gradual fall during the second and third weeks of ontogeny. Competitive binding experiments with unlabeled analogs suggested that both labeled IGFs were binding to type I IGF receptors, and insulin interacted with them. The temperature and pH dependence were relatively higher than those for some other known IGF receptors. We have previously reported that [125I]insulin binding to brain is barely detectable on day 3 and shows a progressive rise throughout the rest of embryonic life. The pattern of IGF and insulin receptors appears to be organ specific, since nonneural tissues such as heart, liver, and limb buds showed different binding profiles in ontogeny. We conclude from these data that IGF receptors develop in chick embryo brain before insulin receptors and probably can mediate effects of IGF and insulin at early stages of embryogenesis.
生长调节素和胰岛素对体外成肌细胞分化的影响。
影响因子：
2.7
作者：
Ewton,DZ;Florini,JR
通讯作者：
Florini,JR
DOI：
10.1016/s0021-9258(18)34631-3
发表时间：
1982-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Massagué;M. Czech
通讯作者：
J. Massagué;M. Czech
亲和标记的生长调节素 C 受体和来自人类胎儿的结合蛋白。
DOI：
10.1210/jcem-58-3-535
发表时间：
1984
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Grizzard,JD;D'Ercole,AJ;Wilkins,JR;Moats-Staats,BM;Williams,RW
通讯作者：
Williams,RW