Characterization of Resistance Patterns and Detection of Apramycin Resistance Genes in Escherichia coli Isolated from Chicken Feces and Houseflies after Apramycin Administration.
Characterization of Resistance Patterns and Detection of Apramycin Resistance Genes in Escherichia coli Isolated from Chicken Feces and Houseflies after Apramycin Administration.
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鸡粪和家蝇给予安普霉素后分离的大肠杆菌耐药模式特征及安普霉素耐药基因检测
DOI：
10.3389/fmicb.2018.00328
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Zhang A;Li Y;Guan Z;Tuo H;Liu D;Yang Y;Xu C;Lei C;Wang H
本研究的目的是评估安普霉素给药对大肠杆菌耐药性发展的影响。大肠杆菌）菌株分离的鸡粪和家蝇在田间条件下。给药组（n = 25，000）的鸡从第1天至第5天在其饮用水中给予连续预防性剂量（0.5 mg/l）的安普霉素，而未给药组（n = 25，000）的饮用水中不添加抗生素。40多天，共收集到1170株E.大肠杆菌菌株从来自同一养鸡场的用药和未用药鸡和家蝇的粪便样品中分离。安普霉素对大肠杆菌的MIC 90值。与第0天（8 μg/ml）相比，从给药组获得的大肠杆菌菌株从第2天至第6天（256-1024 μg/ml）增加了32-128倍。从未给药鸡和家蝇中分离的菌株在第一周内的MIC 90值始终较低（8-16 μg/ml），但从第8天至第10天显示出显著增加（128-1024 μg/ml）。在大肠杆菌中检测到安普霉素抗性基因aac（3）-IV。大肠杆菌菌株来自药物组（n = 71）、未药物组（n = 32）和家蝇组（n = 42）。aac（3）-IV阳性菌株经脉冲场凝胶电泳（PFGE）可分为12型。A型、E型和G型PFGE在药物组和家蝇组均占优势，提示家蝇在E.大肠杆菌抗性菌株。综上所述，我们的研究表明，安普霉素管理可以促进安普霉素耐药E。coli和安普霉素抗性基因acc（3）-IV。反过来，这些菌株可以通过家蝇传播，从而增加了传播多重耐药大肠杆菌的潜在风险。大肠杆菌。
The aim of this study was to evaluate the influence of apramycin administration on the development of antibiotic resistance in Escherichia coli (E. coli) strains isolated from chicken feces and houseflies under field conditions. Chickens in the medicated group (n = 25,000) were given successive prophylactic doses (0.5 mg/l) of apramycin in their drinking water from Days 1 to 5, while no antibiotics were added to the un-medicated groups drinking water (n = 25,000). Over 40 days, a total of 1170 E. coli strains were isolated from fecal samples obtained from medicated and un-medicated chickens and houseflies from the same chicken farm. Apramycin MIC90 values for E. coli strains obtained from the medicated group increased 32–128 times from Days 2 to 6 (256–1024 μg/ml) when compared to those on Day 0 (8 μg/ml). Strains isolated from un-medicated chickens and houseflies had consistently low MIC90 values (8–16 μg/ml) during the first week, but showed a dramatic increase from Days 8 to 10 (128–1024 μg/ml). The apramycin resistance gene aac(3)-IV was detected in E. coli strains from medicated (n = 71), un-medicated (n = 32), and housefly groups (n = 42). All strains positive for aac(3)-IV were classified into 12 pulsed-field gel electrophoresis (PFGE) types. PFGE types A, E, and G were the predominant types in both the medicated and housefly groups, suggesting houseflies play an important role in spreading E. coli-resistant strains. Taken together, our study revealed that apramycin administration could facilitate the occurrence of apramycin-resistant E. coli and the apramycin resistance gene acc(3)-IV. In turn, these strains could be transmitted by houseflies, thus increasing the potential risk of spreading multi-drug-resistant E. coli to the public.
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影响因子：
3.3
作者：
Ng, LK;Martin, I;Mulvey, M
通讯作者：
Mulvey, M
影响因子：
5.2
作者：
Schink, Anne-Kathrin;Kadlec, Kristina;Schwarz, Stefan
通讯作者：
Schwarz, Stefan
影响因子：
5.2
作者：
Dallenne, Caroline;Da Costa, Anaelle;Arlet, Guillaume
通讯作者：
Arlet, Guillaume
影响因子：
4.9
作者：
Keyes, K;Hudson, C;Lee, MD
通讯作者：
Lee, MD
影响因子：
2.6
作者：
Literak, Ivan;Dolejska, Monika;Klimes, Jiri
通讯作者：
Klimes, Jiri