Lilium regale Wilson WRKY2 Regulates Chitinase Gene Expression During the Response to the Root Rot Pathogen Fusarium oxysporum.
Lilium regale Wilson WRKY2 Regulates Chitinase Gene Expression During the Response to the Root Rot Pathogen Fusarium oxysporum.
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百合 Wilson WRKY2 在对根腐病病原体尖孢镰刀菌反应期间调节几丁质酶基因表达
DOI：
10.3389/fpls.2021.741463
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发表时间：
2021
影响因子：
5.6
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Li S;Hai J;Wang Z;Deng J;Liang T;Su L;Liu D
百合根腐病是百合的主要病害，主要由尖孢镰刀菌（Fusarium oxysporum）引起。生产WRKY转录因子在植物免疫应答过程中发挥重要作用。为了阐明WRKY转录因子在植物抗病反应中的作用，从野生百合中克隆了WRKY基因（LrWRKY 2）。尖孢菌属LrWRKY 2编码一种核蛋白，其表达可被多种激素（茉莉酸甲酯、乙烯利、水杨酸和过氧化氢）和F.尖孢菌感染在本研究中，LrWRKY 2过表达的转基因烟草植株对F.与野生型植物相比。此外，茉莉酸生物合成途径相关基因（NtAOC、NtAOS、NtKAT、NtPACX、NtJMT、NtOPR和NtLOX）、病程相关基因（NtCHI、NtGlu 2和NtPR-1）和抗氧化胁迫相关超氧化物歧化酶基因（NtSOD、NtCu-ZnSOD和MnSOD）在转LrWRKY 2基因烟草中的表达水平显著上调。此外，靶向LrWRKY 2的发夹RNA的瞬时表达增加了L. regale音阶到F.尖孢菌属此外，F.从L.盛宴电泳迁移率变动分析表明，LrWRKY 2可以结合到含有W-box元件的LrCHI 2启动子。酵母单杂交实验结果表明LrWRKY 2能够激活LrCHI 2的转录。对用LrWRKY 2和LrCHI 2启动子转化的转基因烟草的检查显示，LrWRKY 2激活LrCHI 2启动子。因此，在L. regale，LrWRKY 2是一个重要的正调控因子，参与植物对F.尖孢霉通过调节LrCHI 2的表达。
Root rot, mainly caused by Fusarium oxysporum, is the most destructive disease affecting lily (Lilium spp.) production. The WRKY transcription factors (TFs) have important roles during plant immune responses. To clarify the effects of WRKY TFs on plant defense responses to pathogens, a WRKY gene (LrWRKY2) was isolated from Lilium regale Wilson, which is a wild lily species highly resistant to F. oxysporum. The expression of LrWRKY2, which encodes a nuclear protein, is induced by various hormones (methyl jasmonate, ethephon, salicylic acid, and hydrogen peroxide) and by F. oxysporum infection. In this study, LrWRKY2-overexpressing transgenic tobacco plants were more resistant to F. oxysporum than the wild-type plants. Moreover, the expression levels of jasmonic acid biosynthetic pathway-related genes (NtAOC, NtAOS, NtKAT, NtPACX, NtJMT, NtOPR, and NtLOX), pathogenesis-related genes (NtCHI, NtGlu2, and NtPR-1), and antioxidant stress-related superoxide dismutase genes (NtSOD, NtCu-ZnSOD, and MnSOD) were significantly up-regulated in LrWRKY2 transgenic tobacco lines. Additionally, the transient expression of a hairpin RNA targeting LrWRKY2 increased the susceptibility of L. regale scales to F. oxysporum. Furthermore, an F. oxysporum resistance gene (LrCHI2) encoding a chitinase was isolated from L. regale. An electrophoretic mobility shift assay demonstrated that LrWRKY2 can bind to the LrCHI2 promoter containing the W-box element. Yeast one-hybrid assay results suggested that LrWRKY2 can activate LrCHI2 transcription. An examination of transgenic tobacco transformed with LrWRKY2 and the LrCHI2 promoter revealed that LrWRKY2 activates the LrCHI2 promoter. Therefore, in L. regale, LrWRKY2 is an important positive regulator that contributes to plant defense responses to F. oxysporum by modulating LrCHI2 expression.
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影响因子：
6.5
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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DOI：
10.1186/s12284-018-0199-0
发表时间：
2018-01-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Lee H;Cha J;Choi C;Choi N;Ji HS;Park SR;Lee S;Hwang DJ
通讯作者：
Hwang DJ