Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques.
Exosomes as Powerful Engines in Cancer: Isolation, Characterization and Detection Techniques.
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外泌体作为癌症的强大引擎：隔离，表征和检测技术。
DOI：
10.3390/bios11120518
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发表时间：
2021-12-16
期刊：
影响因子：
--
通讯作者：
Dong WJ
中科院分区：
文献类型：
--
作者：
Saad MG;Beyenal H;Dong WJ
外泌体是从几乎所有类型的活细胞释放的强大的细胞外纳米囊泡，被认为是控制和重新编程社区内或不同社区之间靶细胞内生理通路的通信引擎（信使）。这些细胞外囊泡的细胞样结构为其蛋白质和DNA/RNA货物提供了保护环境，这些蛋白质和DNA/RNA货物可作为许多恶性疾病的生物标志物，包括传染病和癌症。癌症来源的外泌体控制癌症转移、预后和发展。除了外泌体的独特结构之外，它们的纳米尺寸和与细胞相互作用的倾向使它们成为可行的新型药物递送解决方案。近年来，为了诊断、监测和治疗目的，已经进行了许多研究努力来量化和表征疾病来源的外泌体。本文旨在（1）将外泌体生物标志物与它们的起源联系起来，（2）关注当前的分离和检测方法，（3）讨论和评估外泌体研究用于癌症治疗的拟议技术，（4）对当前外泌体研究的前景进行总结。
Exosomes, powerful extracellular nanovesicles released from almost all types of living cells, are considered the communication engines (messengers) that control and reprogram physiological pathways inside target cells within a community or between different communities. The cell-like structure of these extracellular vesicles provides a protective environment for their proteins and DNA/RNA cargos, which serve as biomarkers for many malicious diseases, including infectious diseases and cancers. Cancer-derived exosomes control cancer metastasis, prognosis, and development. In addition to the unique structure of exosomes, their nanometer size and tendency of interacting with cells makes them a viable novel drug delivery solution. In recent years, numerous research efforts have been made to quantify and characterize disease-derived exosomes for diagnosis, monitoring, and therapeutic purposes. This review aims to (1) relate exosome biomarkers to their origins, (2) focus on current isolation and detection methods, (3) discuss and evaluate the proposed technologies deriving from exosome research for cancer treatment, and (4) form a conclusion about the prospects of the current exosome research.
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DOI：
10.1007/978-1-61779-959-4_21
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Conde-Vancells, Javier;Falcon-Perez, Juan M
通讯作者：
Falcon-Perez, Juan M
影响因子：
19.6
作者：
Alvarez, M. Lucrecia;Khosroheidari, Mahdieh;DiStefano, Johanna K.
通讯作者：
DiStefano, Johanna K.
影响因子：
4
作者：
Colombo, Marina;Moita, Catarina;Raposo, Graca
通讯作者：
Raposo, Graca
影响因子：
4.3
作者：
Campuzano, Susana;Pedrero, Maria;Pingarron, Jose M.
通讯作者：
Pingarron, Jose M.
影响因子：
3.3
作者：
Coren, Lori V.;Shatzer, Teresa;Ott, David E.
通讯作者：
Ott, David E.