Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes.
Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes.
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表达报告基因的可复制ΔNS1甲型流感病毒。
DOI：
10.3390/v13040698
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发表时间：
2021-04-17
期刊：
影响因子：
--
通讯作者：
Martinez-Sobrido L
中科院分区：
文献类型：
--
作者：
Nogales A;Schotsaert M;Rathnasinghe R;DeDiego ML;García-Sastre A;Martinez-Sobrido L
甲型流感病毒（IAV）能够感染多种哺乳动物和禽类物种，并且在人类中，IAV是造成呼吸道疾病的年度季节性流行和偶尔大流行的原因，具有显著的健康和经济影响。研究IAV涉及费力的二级方法来识别感染的细胞。因此，为了规避这一要求，近年来，已经开发了表达可追踪报告基因的多个可复制的感染性IAV。这些IAV对于病毒复制的体外和/或体内研究、中和抗体或抗病毒剂的鉴定以及评价疫苗功效的研究等非常有用。在本报告中，我们首次描述了两种可复制的甲型流感/波多黎各/8/1934 H1N1（PR 8）病毒的产生和表征，其中病毒非结构蛋白1（NS 1）被单体（m）Cherry荧光蛋白或NanoLuc荧光素酶（Nluc）蛋白取代。Δ NS 1 mCherry能够在培养细胞和信号转导和转录激活因子1（STAT 1）缺陷小鼠中复制，尽管程度低于表达相同mCherry荧光蛋白的野生型（WT）PR 8病毒（WT mCherry）。值得注意的是，通过荧光显微镜或荧光素酶读板仪分别在感染的细胞中检测到报告基因（mCherry或Nluc）的表达。表达报告基因的Δ NS 1 IAV为更好地了解IAV的生物学和发病机制提供了新的途径，并代表了开发新的抗IAV感染治疗方法的极好工具。
The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts. Studying IAV involves laborious secondary methodologies to identify infected cells. Therefore, to circumvent this requirement, in recent years, multiple replication-competent infectious IAV expressing traceable reporter genes have been developed. These IAVs have been very useful for in vitro and/or in vivo studies of viral replication, identification of neutralizing antibodies or antivirals, and in studies to evaluate vaccine efficacy, among others. In this report, we describe, for the first time, the generation and characterization of two replication-competent influenza A/Puerto Rico/8/1934 H1N1 (PR8) viruses where the viral non-structural protein 1 (NS1) was substituted by the monomeric (m)Cherry fluorescent or the NanoLuc luciferase (Nluc) proteins. The ΔNS1 mCherry was able to replicate in cultured cells and in Signal Transducer and Activator of Transcription 1 (STAT1) deficient mice, although at a lower extent than a wild-type (WT) PR8 virus expressing the same mCherry fluorescent protein (WT mCherry). Notably, expression of either reporter gene (mCherry or Nluc) was detected in infected cells by fluorescent microscopy or luciferase plate readers, respectively. ΔNS1 IAV expressing reporter genes provide a novel approach to better understand the biology and pathogenesis of IAV, and represent an excellent tool to develop new therapeutic approaches against IAV infections.
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影响因子：
4.4
作者：
Carrero JA
通讯作者：
Carrero JA
影响因子：
3.7
作者：
Breen M;Nogales A;Baker SF;Perez DR;Martínez-Sobrido L
通讯作者：
Martínez-Sobrido L
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影响因子：
4.8
作者：
Dankar SK;Miranda E;Forbes NE;Pelchat M;Tavassoli A;Selman M;Ping J;Jia J;Brown EG
通讯作者：
Brown EG
影响因子：
5.2
作者：
Ferhadian D;Contrant M;Printz-Schweigert A;Smyth RP;Paillart JC;Marquet R
通讯作者：
Marquet R
影响因子：
2.7
作者：
Choi, Eun-hye;Song, Min-Suk;Choi, Young Ki
通讯作者：
Choi, Young Ki