Mechanically stable surface-hydrophobilized chitosan nanofibrous barrier membranes for guided bone regeneration
Mechanically stable surface-hydrophobilized chitosan nanofibrous barrier membranes for guided bone regeneration
复制标题
用于引导骨再生的机械稳定表面疏水化壳聚糖纳米纤维屏障膜
DOI：
10.1088/1748-605x/aa853c
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
WU CHAOXI
中科院分区：
文献类型：
--
作者：
WU CHAOXI
基于壳聚糖的生物膜在引导性骨再生（GBR）中的应用受到其在水性环境中不受控制的溶胀和机械不稳定性的限制。本文介绍了表面丁酰化壳聚糖膜（BCSNF）的稳定性和性能的显着改善，大大提高了它们在GBR中的潜力。BCSNF膜的总取代度为1.61，平均直径为99.3 ± 33.7 nm，溶胀度降低75%，与水性环境中的未改性纤维相比，缝线拔出强度约增加一倍。在5周的磷酸盐缓冲盐水-溶菌酶降解研究中，发现BCSNF膜的剩余质量分数比未改性的纤维多11.5%。在体外，发现BCSNF膜支持成纤维细胞的粘附和增殖，并且是细胞遮蔽的。在体内，发现BCSNF膜在12周愈合期内显著改善大鼠颅骨临界尺寸缺损的再生，并且显示出比市售胶原蛋白膜更好的屏障功能，几乎没有软组织穿透膜。总之，这些数据为在GBR应用中使用BCSNF膜提供了强有力的科学证据。
The use of chitosan based nanofiber membranes in guided bone regeneration (GBR) is limited by its uncontrolled swelling and mechanical instability in aqueous environments. This paper describes the significantly improved stability and properties of surface butyrylated chitosan nanofiber (BCSNF) membranes that greatly enhance their potential in GBR. The BCSNF membranes exhibited an overall degree of substitution of 1.61, an average diameter of 99.3 ± 33.7 nm, and a 75% decrease in swelling with an approximate doubling in suture pull out strengths as compared to unmodified fibers in aqueous environment. In a five week phosphate-buffered saline–lysozyme degradation study, it was found that the remaining mass fraction of BCSNF membranes was 11.5% more than that of unmodified fibers. In vitro, the BCSNF membranes were found to support the adhesion and proliferation of fibroblasts and were cell occulusive. In vivo, the BCSNF membranes were found to significantly improve the regeneration of a rat calvarial critical size defect in a 12 week healing period and showed better barrier function than commercially available collagen membranes with little soft tissue penetration through the membranes. Taken together, these data provide strong scientific evidence for use of BCSNF membranes in GBR applications.
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DOI：
10.1002/jbm.b.33430
发表时间：
2016-04
期刊：
Journal of biomedical materials research. Part B, Applied biomaterials
影响因子：
--
作者：
Rowe MJ;Kamocki K;Pankajakshan D;Li D;Bruzzaniti A;Thomas V;Blanchard SB;Bottino MC
通讯作者：
Bottino MC
影响因子：
2.1
作者：
Goh, V.;Hackmack, P. P.;Leung, W. K.
通讯作者：
Leung, W. K.
影响因子：
5.7
作者：
Chaoxi Wu;H. Su;Shunqing Tang;J. Bumgardner
通讯作者：
Chaoxi Wu;H. Su;Shunqing Tang;J. Bumgardner
影响因子：
--
作者：
M. Schwartzmann
通讯作者：
M. Schwartzmann
影响因子：
11.2
作者：
Ma, Guiping;Yang, Dongzhi;Nie, Jun
通讯作者：
Nie, Jun