Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells.
Visual inspection versus quantitative flow cytometry to detect aberrant CD2 expression in malignant T cells.
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DOI：
10.1002/cyto.b.20507
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发表时间：
2010-05
影响因子：
3.4
通讯作者：
Stetler-Stevenson, Maryalice
中科院分区：
文献类型：
--
作者：
Arun, Indu;Wulu, Jacqueline A.;Janik, John E.;Jasper, Gregory A.;Yuan, Constance M.;Venzon, David;Stetler-Stevenson, Maryalice
T细胞抗原表达水平异常发生在T细胞瘤形成中。我们检测了恶性和正常T细胞中的CD 2表达，以确定CD 2表达水平是否存在显著差异，以及是否有助于定量检测这种差异。对36例成熟T细胞瘤患者的标本进行流式细胞术免疫表型（FCI）评价。基于异常FCI和形态学鉴定异常T细胞。使用抗CD 2和QuantiBRITE珠标准品的1：1 PE缀合物定量CD 2表达水平，以计算每个细胞结合的抗体（ABC）。比较ABC测量与简单检查点图的有效性。在成熟T细胞恶性肿瘤中经常观察到CD 2表达水平异常。CD 2 ABC值在检测恶性和正常T细胞之间的差异方面高度敏感（p=0.0028）。在大多数情况下（24/32份标本，75%），CD 2 ABC差异> 20%。CD 2 ABC在正常T细胞中具有高变异性。通过CD 2 ABC定量，恶性T细胞的CD 2表达与正常T细胞的CD 2表达显著不同。正常T细胞CD 2 ABC的高变异性限制了正常参考范围的确定，从而限制了其在T细胞瘤形成诊断中的实用性。然而，当存在残留的正常T细胞进行比较时，CD 2的检查可以帮助检测肿瘤细胞。此外，CD 2定量的灵敏度增加在确认FCI病例中是有价值的，其中CD 2表达的异常仅通过目视检查难以理解。
Abnormal levels of T cell antigen expression occur in T cell neoplasia. We examined CD2 expression in malignant and normal T cells to determine if the level of CD2 expression differed significantly and if quantitation assisted in detecting this difference. Flow cytometric immunophenotypic (FCI) evaluation was performed on specimens from 36 patients with mature T cell neoplasia. Abnormal T cells were identified based upon abnormal FCI and morphology. Levels of CD2 expression were quantitated using 1:1 PE conjugates of anti-CD2 and QuantiBRITE bead standards to calculate the antibodies bound per cell (ABC). The efficacy of ABC measurement verses simple examination of dots plots was compared. Abnormal levels of CD2 expression were frequently observed in mature T cell malignancies. The CD2 ABC values were highly sensitive in detecting differences between malignant and normal T cells (p=0.0028). In most cases (24/32 specimens, 75%) CD2 ABCs differed by > 20%. CD2 ABCs had high variability in normal T cells. CD2 expression by malignant T cells differed significantly from that of normal T-cells by CD2 ABC quantitation. The high variability in normal T cell CD2 ABCs limited the determination of normal reference ranges, and thus its utility in the diagnosis of T cell neoplasia. However, examination of CD2 can help in detection of tumor cells when residual normal T cells are present for comparison. Moreover, the increased sensitivity of CD2 quantitation is valuable in confirming FCI cases where abnormalities in CD2 expression are difficult to appreciate by visual inspection alone.
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