In vivo fluorescence imaging with high-resolution microlenses.
In vivo fluorescence imaging with high-resolution microlenses.
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DOI:
10.1038/nmeth.1339
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发表时间:
2009-07
期刊:
影响因子:
48
通讯作者:
Schnitzer, Mark J.
中科院分区:
文献类型:
--
作者:
Barretto, Robert P. J.;Messerschmidt, Bernhard;Schnitzer, Mark J.
Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to conventional microscopy. Here we describe high-resolution microlenses, and illustrate two-photon imaging of dendritic spines on hippocampal neurons and dual-color nonlinear optical imaging of neuromuscular junctions in live mice.
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影响因子:
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通讯作者:
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