Characterization of diet-dependent temporal changes in circulating short-chain fatty acid concentrations: A randomized crossover dietary trial.
Characterization of diet-dependent temporal changes in circulating short-chain fatty acid concentrations: A randomized crossover dietary trial.
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DOI：
10.1093/ajcn/nqab211
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发表时间：
2022-11
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
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从食品中生产SCFAs是一个复杂的动态糖酵解发酵过程，由人类和肠道微生物因素介导。缺乏对SCFA产生以及SCFA概况与饮食模式之间关系的了解。使用一种新的GC-MS方法研究了响应于2种对比饮食的SCFA浓度的时间变化。样本来自一项随机、对照、交叉试验，旨在表征对4种饮食的代谢反应。参与者（n = 19）在住院期间（72小时）进行这些饮食。在第3天的早餐后（AB）、午餐后（AL）和晚餐后（AD）2小时采集血清样品，并在第4天获得空腹样品（FA）。在第3天收集24小时尿液样本。在这项子研究中，使用定制的GC-MS方法分析了代表高度遵守WHO健康饮食建议的饮食和典型西方饮食的2种极端饮食的样本，该方法用于检测和定量血清和尿液样本中的10种SCFA和前体。在所有时间点观察到血清SCFA浓度存在相当大的个体间差异，并且在两种饮食中观察到时间波动。尽管样本采集时间对循环SCFA浓度有较大影响，但不健康饮食与乙酸浓度较低有关（FA：系数：-17.0; SE：5.8; P趋势= 0.00615），2-甲基丁酸（AL：系数：-0.1; SE：0.028; P趋势= 4.13 × 10-4和AD：系数：-0.1; SE：0.028; P趋势= 2.28 × 10-3）和2-羟基丁酸（FA：系数：-15.8; SE：5.11; P趋势：4.09 × 10-3）。与此相反，不健康饮食中的乳酸显著较高（AL：系数：750.2; SE：315.2; P-trend = 0.024和AD：系数：1219.3; SE：322.6; P-trend：8.28 × 10-4）。GC-MS方法允许对受饮食影响的SCFA浓度的昼夜模式进行强有力的绘图，并强调了在饮食研究中标准化SCFA测量时间的重要性。该试验在NIHR UK临床试验网关上注册，ISRCTN编号为ISRCTN 43087333。
Production of SCFAs from food is a complex and dynamic saccharolytic fermentation process mediated by both human and gut microbial factors. Knowledge of SCFA production and of the relation between SCFA profiles and dietary patterns is lacking. Temporal changes in SCFA concentrations in response to 2 contrasting diets were investigated using a novel GC-MS method. Samples were obtained from a randomized, controlled, crossover trial designed to characterize the metabolic response to 4 diets. Participants (n = 19) undertook these diets during an inpatient stay (of 72 h). Serum samples were collected 2 h after breakfast (AB), after lunch (AL), and after dinner (AD) on day 3, and a fasting sample (FA) was obtained on day 4. The 24-h urine samples were collected on day 3. In this substudy, samples from the 2 extreme diets representing a diet with high adherence to WHO healthy eating recommendations and a typical Western diet were analyzed using a bespoke GC-MS method developed to detect and quantify 10 SCFAs and precursors in serum and urine samples. Considerable interindividual variation in serum SCFA concentrations was observed across all time points, and temporal fluctuations were observed for both diets. Although the sample collection timing exerted a greater magnitude of effect on circulating SCFA concentrations, the unhealthy diet was associated with a lower concentration of acetic acid (FA: coefficient: –17.0; SE: 5.8; P-trend = 0.00615), 2-methylbutyric acid (AL: coefficient: –0.1; SE: 0.028; P-trend = 4.13 × 10–4 and AD: coefficient: –0.1; SE: 0.028; P-trend = 2.28 × 10–3), and 2-hydroxybutyric acid (FA: coefficient: –15.8; SE: 5.11; P-trend: 4.09 × 10–3). In contrast, lactic acid was significantly higher in the unhealthy diet (AL: coefficient: 750.2; SE: 315.2; P-trend = 0.024 and AD: coefficient: 1219.3; SE: 322.6; P-trend: 8.28 × 10–4). The GC-MS method allowed robust mapping of diurnal patterns in SCFA concentrations, which were affected by diet, and highlighted the importance of standardizing the timing of SCFA measurements in dietary studies. This trial was registered on the NIHR UK clinical trial gateway and with ISRCTN as ISRCTN43087333.
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影响因子：
9.8
作者：
FRAYN, KN;COPPACK, SW;EVANS, RD
通讯作者：
EVANS, RD
影响因子：
4.8
作者：
Brown, AJ;Goldsworthy, SM;Dowell, SJ
通讯作者：
Dowell, SJ
影响因子：
29
作者：
De Vadder, Filipe;Kovatcheva-Datchary, Petia;Mithieux, Gilles
通讯作者：
Mithieux, Gilles
DOI：
10.1038/ijo.2015.84
发表时间：
2015-09
期刊：
International journal of obesity (2005)
影响因子：
--
作者：
Byrne CS;Chambers ES;Morrison DJ;Frost G
通讯作者：
Frost G
影响因子：
5.9
作者：
Boets E;Deroover L;Houben E;Vermeulen K;Gomand SV;Delcour JA;Verbeke K
通讯作者：
Verbeke K