Systematic review with meta-analysis of diagnostic test accuracy for COVID-19 by mass spectrometry.
Systematic review with meta-analysis of diagnostic test accuracy for COVID-19 by mass spectrometry.
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DOI：
10.1016/j.metabol.2021.154922
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Bailey MJ
中科院分区：
文献类型：
--
作者：
Spick M;Lewis HM;Wilde MJ;Hopley C;Huggett J;Bailey MJ
全球COVID-19大流行导致许多领域取得了广泛发展，包括用质谱法诊断COVID-19感染。本系统综述和荟萃分析的目的是评估迄今为止在广泛的生物基质中开发的质谱诊断测试的准确性，以及评估迄今发表的研究的偏倚风险和适用性。采用PRISMA-DTA框架的系统评价纳入了23项回顾性观察性队列研究，共有2858名COVID-19阳性参与者和2544名对照组。通过QUADAS-2问卷评估偏倚风险和适用性。除了评估异质性外，还对敏感性、特异性、诊断准确性和约登指数进行了荟萃分析。在本文回顾的研究中，灵敏度平均为0.87（四分位数范围0.81-0.96），特异性为0.88（四分位数范围0.82-0.98），受试者工作特征汇总曲线下面积为0.93。按亚组划分，鼻咽拭子病毒蛋白质组学分析和血浆和血清代谢组学分析获得最佳诊断结果。其他取样基质（呼吸、皮脂、唾液）的性能不太好，表明这些方案目前在临床应用方面还不够成熟。这项系统综述和荟萃分析表明，质谱和组学在获得准确的COVID-19诊断检测结果方面具有潜力，但也强调了在将这些方法转化为临床应用之前，需要进一步努力优化和协调各实验室的实践。质谱法补充了RT-PCR在COVID-19诊断中的应用。质谱诊断COVID-19的荟萃分析显示准确率为87%。质谱在入院诊断中是一个很有前途的用例。未来的工作应该集中在跨实验室验证特征和协议。
The global COVID-19 pandemic has led to extensive development in many fields, including the diagnosis of COVID-19 infection by mass spectrometry. The aim of this systematic review and meta-analysis was to assess the accuracy of mass spectrometry diagnostic tests developed so far, across a wide range of biological matrices, and additionally to assess risks of bias and applicability in studies published to date. 23 retrospective observational cohort studies were included in the systematic review using the PRISMA-DTA framework, with a total of 2858 COVID-19 positive participants and 2544 controls. Risks of bias and applicability were assessed via a QUADAS-2 questionnaire. A meta-analysis was also performed focusing on sensitivity, specificity, diagnostic accuracy and Youden's Index, in addition to assessing heterogeneity. Sensitivity averaged 0.87 in the studies reviewed herein (interquartile range 0.81–0.96) and specificity 0.88 (interquartile range 0.82–0.98), with an area under the receiver operating characteristic summary curve of 0.93. By subgroup, the best diagnostic results were achieved by viral proteomic analyses of nasopharyngeal swabs and metabolomic analyses of plasma and serum. The performance of other sampling matrices (breath, sebum, saliva) was less good, indicating that these protocols are currently insufficiently mature for clinical application. This systematic review and meta-analysis demonstrates the potential for mass spectrometry and ‘omics in achieving accurate test results for COVID-19 diagnosis, but also highlights the need for further work to optimize and harmonize practice across laboratories before these methods can be translated to clinical applications. Mass spectrometry complements RT-PCR in COVID-19 diagnostics. Meta-analysis of mass spectrometry diagnostics for COVID-19 shows accuracy of 87%. Diagnosis in admissions settings is a promising use-case for mass spectrometry. Future work should focus on validating features and protocols across laboratories.
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DOI：
10.1016/j.trac.2021.116328
发表时间：
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期刊：
Trends in analytical chemistry : TRAC
影响因子：
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作者：
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影响因子：
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10.1016/j.jmsacl.2021.09.001
发表时间：
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影响因子：
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