Age-related inflammatory mediators in coronary artery disease (II).
Age-related inflammatory mediators in coronary artery disease (II).
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冠状动脉疾病中与年龄相关的炎症介质（II）。
DOI：
10.1016/j.ijcard.2013.07.157
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Bao,S
中科院分区：
文献类型：
--
作者：
Duflou,J;Zhang,X;Puranik,S;Bao,S
冠状动脉疾病是全球发病率和死亡率的一个主要原因，也是35岁以下澳大利亚人自然猝死的最常见原因之一。尽管炎症是动脉粥样硬化形成的关键过程，但人们对早发性冠心病中炎症的具体调节机制知之甚少。炎症有助于CAD的发展，而细胞因子在早期CAD的发病机制中有特殊的作用。关于新型白细胞介素，如IL-27、-29和IL-31-34的知识已经出现，因为它们与各种炎症疾病状态（如结肠炎、牛皮癣和慢性阻塞性肺病）有关，但关于它们在动脉粥样硬化中的具体作用知之甚少。冠状动脉标本取自2009年至2012年期间在澳大利亚新南威尔士州法医学部门进行的尸检中50例早产儿（≤60岁）和50例成年男性（N60岁）的冠状动脉标本，其中CAD是死因，如前所述[1]。这些病例没有非法使用药物的记录。进一步招募了20名非心源性死亡对照组。本研究严格按照澳大利亚悉尼地方卫生区伦理审查委员会的规定进行。采用H&E和Movat染色，对早期和成熟冠心病患者及对照组的罪魁祸首粥样斑块的冠状动脉切片进行染色，见[3]。进一步对切片进行IL-27、-29和31 - 34染色。兔抗人一抗（Abcam，悉尼，澳大利亚）和HRP山羊抗兔免疫球蛋白（Dako，悉尼，澳大利亚）。阳性染色用Image-Pro®Plus 7拍照并定量，如[2]所述。统计分析采用参数单因素方差分析和Tukey事后检验。统计学显著水平定义为pb0.05为显著结果。
Coronary artery disease (CAD), a major cause of morbidity and mortality globally, is among the most common causes of sudden natural deaths in Australians under the age of 35 years [1]. Although inflammation is a key process in atherogenesis, little is known about specific regulators of inflammation in premature onset CAD. Inflammation contributes to the development of CAD, and cytokines are specifically implicated in the pathogenesis of premature CAD [2]. Knowledge regarding novel interleukins, eg IL-27,-29 and IL-31–34, has emerged as they relate to various inflammatory disease states (eg, colitis, psoriasis, and COPD), but little is known regarding their specific role in atherogenesis.Coronary artery specimens were obtained from 50 premature (≤ 60 y) and 50 mature (N60 y) male decedents where CAD was the cause of death at post-mortems performed at the NSW Department of Forensic Medicine, Australia, between 2009 and 2012, as described previously [2]. These cases had no recorded history of illicit drug use. Twenty non-cardiac-death controls were further recruited. This study was conducted in strict compliance with the Sydney Local Health District Ethics Review Committee, Australia. Coronary artery sections of culprit atheromatous plaque from premature and mature CAD cases and controls were stained with H&E and Movat's, as described [3]. The sections were further stained for IL-27,-29, and-31–34. Each of the polyclonal rabbit anti-human primary antibodies (Abcam, Sydney, Australia) were followed by polymer HRP goat anti-rabbit immunoglobulin (Dako, Sydney, Australia). The positive staining was photographed and quantified using Image-Pro® Plus 7, as described [2]. Statistical analysis was performed by parametric one-way ANOVAs and Tukey post-hoc tests. The levels of statistical significance was defined as pb 0.05 for significant results.
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影响因子：
27.4
作者：
Dinarello, C. A.;Kim, S-H
通讯作者：
Kim, S-H
影响因子：
14.2
作者：
Neis, Mark M.;Peters, Bettina;Hermanns, Heike M.
通讯作者：
Hermanns, Heike M.
DOI：
10.1084/jem.20071868
发表时间：
2008-02-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Miller AM;Xu D;Asquith DL;Denby L;Li Y;Sattar N;Baker AH;McInnes IB;Liew FY
通讯作者：
Liew FY
影响因子：
6.5
作者：
Shibata, Sayaka;Tada, Yayoi;Sato, Shinichi
通讯作者：
Sato, Shinichi
DOI：
10.1097/pas.0b013e31824aae0a
发表时间：
2012-01
期刊：
Medicine, Science and the Law
影响因子：
--
作者：
R. Puranik;C. Chow;Johan Duflou;M. Kilborn;M. McGuire
通讯作者：
R. Puranik;C. Chow;Johan Duflou;M. Kilborn;M. McGuire