Infection of oomycetes and bacteria associated with their specific colocalization in chum salmon eggs
Infection of oomycetes and bacteria associated with their specific colocalization in chum salmon eggs
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卵菌和细菌的感染与其在鲑鱼卵中的特定共定位相关
DOI：
10.1016/j.aquaculture.2021.737244
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Fujikura K
中科院分区：
文献类型：
--
作者：
Orui Sakaguchi S;Ikuta T;Tame A;Shimizu Y;Takishita K;Nagano Y;Kasai H;Fujikura K
为了阐明卵菌（水霉菌）和细菌在一个单一的鸡蛋的鲑鱼，虹鳟鱼，卵菌感染的鸡蛋的本地化进行了研究，通过明场显微镜，扫描电子显微镜（SEM），和荧光原位杂交（FISH）使用卵菌通用和细菌物种特异性探针。此外，使用从用于FISH分析的卵切片提取的基因组DNA，在卵的绒毛膜和细胞质上进行卵菌和细菌的DNA条形码化。利用明场显微镜和扫描电镜观察，卵菌菌丝穿透绒毛膜侵入卵细胞质。基于DNA条形码技术，对两种卵菌（Pythiummonospermum和Pythiumsp. SE-OP 1）和三种细菌物种（Flavobacteriumplurextorum，Flavobacteriumsp. SE-BF 1和Undibacterium pigrum）在感染的鸡蛋中主要被检测到。FISH分析表明卵菌在卵细胞质和卵壳中的定位没有明显差异。相比之下，使用细菌物种特异性探针的FISH分析表明它们的定位存在明显差异。SE-BF 1和U.色素瘤主要分布在绒毛膜内和侵入细胞质的卵菌菌丝周围。这些结果是第一个表明U. pigrum与卵菌有一定的亲缘关系，与卵菌协同入侵卵。然而，黄杆菌属（Flavobacterium sp.）SE-BF 1的入侵机制不同于ofU。pigrum。虽然本研究是初步的，但卵菌与U. pigrum与鲑鱼卵感染相关的研究需要详细阐明，这一发现可能为鲑鱼卵中细菌的感染机制提供新的见解，这是鲑鱼孵化场长期存在的问题。
To elucidate the localization of oomycetes (water molds) and bacteria in a single egg of chum salmon,Oncorhynchus keta, eggs infected with oomycetes were examined by bright-field microscopy, scanning electron microscopy (SEM), and fluorescentin situhybridization (FISH) using oomycete universal and bacterial species-specific probes. Furthermore, DNA barcoding for oomycetes and bacteria was performed on the chorion and cytoplasm of the eggs using genomic DNA extracted from the sections of eggs used for FISH analyses. Using bright-field microscopy and SEM, it was shown that oomycete hyphae penetrated the chorion and invaded the cytoplasm of the eggs. Based on DNA barcoding, two oomycetes (PythiummonospermumandPythiumsp. SE-OP1), and three bacterial species (Flavobacteriumplurextorum,Flavobacteriumsp. SE-BF1 andUndibacterium pigrum) were predominantly detected in the infected eggs. FISH analyses indicated that localization of the oomycetes was not noticeable different between the chorion and cytoplasm of the eggs. In contrast, FISH analyses using bacterial species-specific probes demonstrated clear differences in their localization.Flavobacteriumsp. SE-BF1 andU. pigrumwere predominantly found inside the chorion and around the oomycete hyphae invading the cytoplasm, respectively. These results are the first to indicate the possibility thatU. pigrumhas a specific relationship with oomycetes and coordinately invades eggs with oomycetes. However, it seems thatFlavobacteriumsp. SE-BF1 invades by a mechanism different from that ofU. pigrum. Although this study is preliminary and the interaction between oomycetes andU. pigrumassociated with chum salmon egg infection needs to be elucidated in detail, this finding may provide new insights into the infection mechanism of bacteria in salmon eggs, which is a longstanding problem in salmon hatcheries.
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