Identification and characterization of intrahepatic hepatitis b virus dna in hbsag‐seronegative patients with chronic liver disease and hepatocellular carcinoma in Taiwan
Identification and characterization of intrahepatic hepatitis b virus dna in hbsag‐seronegative patients with chronic liver disease and hepatocellular carcinoma in Taiwan
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台湾 hbsag 血清阴性慢性肝病和肝细胞癌患者肝内乙型肝炎病毒 DNA 的鉴定和特征分析
DOI：
10.1002/hep.1840120321
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发表时间：
1990
期刊：
影响因子：
13.5
通讯作者：
Ding‐Shinn Chen
中科院分区：
文献类型：
--
作者：
M. Lai;Pei‐Jer Chen;P. Yang;J. Sheu;J. Sung;Ding‐Shinn Chen
为探讨乙肝病毒感染在慢性肝病及肝细胞癌患者中的作用，我们用Southern杂交方法检测了112例慢性肝病及肝细胞癌患者肝活检组织中的乙肝病毒DNA。研究对象包括43例非酒精性慢性肝病患者、21例肝细胞癌患者和48例其他肝胆和胃肠道疾病的对照患者。为了证实肝内乙肝病毒DNA信号的特异性和了解整合的病毒序列的结构，对1例患者进行了整合的乙肝病毒DNA的分子克隆和DNA测序。在13例既往无乙肝病毒感染血清学证据的患者中，在肝脏中未发现乙肝病毒序列。在其他有乙肝病毒暴露史的HBs Ag阴性患者中，肝内乙肝病毒DNA的阳性率很高(7%)。肝内乙肝病毒DNA均为整合型。非酒精性慢性肝炎、肝硬化组阳性率(2%)与其他肝胆、胃肠道疾病对照组(4%)比较差异无统计学意义。然而，肝细胞癌患者与非肝细胞癌患者的乙肝病毒DNA整合率差异有统计学意义(19%vs.3%，p<；0.05)。对一例抗-HBs阳性患者的整合乙肝病毒DNA的3.0kb EcoRI片段进行了分子克隆和测序，结果表明该片段是乙肝病毒基因组的部分拷贝。整合的病毒序列的广泛重排被发现，这可能是不产生HIBsAg的原因。我们的结论是，在台湾慢性肝病及肝细胞癌患者中，相当比例(7.8%)的慢性肝病及肝细胞癌患者的肝脏中含有乙肝病毒DNA序列，乙肝病毒DNA的整合可能在肝细胞癌的发生发展中起作用。(《肝病》1990；12：575-581)。
To clarify the role of hepatitis B virus infection in HBsAg‐seronegative patients with chronic liver disease and hepatocellular carcinoma in Taiwan, we examined the hepatitis B virus DNA in liver biopsy tissues of 112 patients by Southern blot analysis. The patients studied included 43 patients with nonalcoholic chronic liver disease, 21 patients with hepatocellular carcinoma and 48 control patients with other hepatobiiary and gastrointestinal diseases. To confirm the specificity of the intrahepatic hepatitis B virus DNA signal and to understand the structure of the integrated viral sequences, molecular cloning and DNA sequencing of an integrated hepatitis B virus DNA were done in one patient. Among 13 patients without serological evidence of previous hepatitis B virus infection, no hepatitis B virus sequences were found in the liver. In other HBsAg‐negative patients with evidence of previous hepatitis B virus exposure, a substantial positive rate of intrahepatic hepatitis B virus DNA was found (7%). The intrahepatic hepatitis B virus DNA was all in integrated form. The positive rate among patients with nonalcoholic chronic hepatitis and cirrhosis (2%) was not different from that of the control group with other hepatobiliary and gastrointestinal diseases (4%). However, the positive rate of integrated hepatitis B virus DNA between hepatocellular carcinoma patients and nonhepatocellular carcinoma patients was statistically significant (19% vs. 3%, p <0.05). Molecular cloning and sequencing of a 3.0 kb EcoRI fragment of an integrated hepatitis B virus DNA from an anti‐HBspositive patient revealed that it was a partial copy of the hepatitis B virus genome. Extensive rearrangement of the integrated viral sequences was found and might account for nonproduction of HIBsAg. We conclude that a substantial (7.8%) proportion of HBsAg‐negative patients with chronic liver disease and hepatocellular carcinoma in Taiwan have hepatitis B virus DNA sequence in the liver, and integration of hepatitis B virus DNA may play a role in the development of hepatocellular carcinoma. (HEPATOLOGY 1990;12:575–581).
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影响因子：
56.9
作者：
BISHOP, JM
通讯作者：
BISHOP, JM
影响因子：
158.5
作者：
BRECHOT, C;DEGOS, F;BERTHELOT, P
通讯作者：
BERTHELOT, P
乙型肝炎病毒相关变种的鉴定和传播。
DOI：
10.1073/pnas.83.17.6608
发表时间：
1986
影响因子：
11.1
作者：
Wands,JR;Fujita,YK;Isselbacher,KJ;Degott,C;Schellekens,H;Dazza,MC;Thiers,V;Tiollais,P;Brechot,C
通讯作者：
Brechot,C
影响因子：
158.5
作者：
SHAFRITZ, DA;SHOUVAL, D;KEW, MC
通讯作者：
KEW, MC
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S