A Cross-Sectional Study of Dairy Cattle Metagenomes Reveals Increased Antimicrobial Resistance in Animals Farmed in a Heavy Metal Contaminated Environment.
A Cross-Sectional Study of Dairy Cattle Metagenomes Reveals Increased Antimicrobial Resistance in Animals Farmed in a Heavy Metal Contaminated Environment.
复制标题
DOI：
10.3389/fmicb.2020.590325
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Ganda E
中科院分区：
文献类型：
--
作者：
Gaeta NC;Bean E;Miles AM;de Carvalho DUOG;Alemán MAR;Carvalho JS;Gregory L;Ganda E
在经济和社会发展中使用重金属会在环境中造成有毒废物的堆积。高浓度的重金属会损害人类和动物的健康，导致抗生素耐药性的产生，并可能改变牛的微生物区系。研究食品中重金属对人体健康的影响，对于确定环境重金属污染对人类健康的潜在危害具有重要意义。2015年，由于一座采矿大坝决口，4300万立方米的铁矿石废料流入了巴西马里亚纳市周围的多斯河流域。在这场环境灾难之后，我们调查了长期接触受污染的饮用水对奶牛微生物群和抵抗力的影响。在环境灾难发生4年后，我们在16头奶牛的粪便、瘤胃液和鼻咽中发现了细菌抗菌素耐药性(AMR)基因。牛一直暴露在重金属污染的水中，直到样本采集(A)，并将它们与356公里外一个未受影响的农场的16头奶牛的类似样本进行比较(B)。A农场和B农场的微生物群和抗性菌群在许多方面存在差异。黄牛鼻咽、瘤胃和粪便中存在的与AMR相关的基因分布具有高度的异质性，大多数基因只存在于少数样本中。A农场AMR基因的相对丰度和流行率(存在/不存在)均高于B农场。来自A农场的样本对多种药物、金属、杀虫剂和多药耐药的抗性基因的流行率较高。粪便AMR基因相对丰度较高，瘤胃液次之，鼻咽AMR基因相对丰度最低。元基因组功能注释表明，重金属暴露的选择性压力可能会使途径多样性向更少、更专门的功能倾斜。这是第一项评估巴西环境事故后果的研究，矿坝坍塌在奶牛的微生物群中。我们的发现表明，重金属在环境中的长期存在可能会导致微生物区系的差异和抗菌素耐药性细菌的浓缩。我们的结果还表明，与细菌读数相对较低的瘤胃和鼻咽样本相比，AMR基因在粪便样本中最容易检测到。由于重金属污染对动物微生物群有影响，因此有必要进行环境管理，以保护食品系统免受危险后果的影响。
The use of heavy metals in economic and social development can create an accumulation of toxic waste in the environment. High concentrations of heavy metals can damage human and animal health, lead to the development of antibiotic resistance, and possibly change in bovine microbiota. It is important to investigate the influence of heavy metals in food systems to determine potential harmful effects environmental heavy metal contamination on human health. Because of a mining dam rupture, 43 million cubic meters of iron ore waste flowed into the Doce river basin surrounding Mariana City, Brazil, in 2015. Following this environmental disaster, we investigated the consequences of long-term exposure to contaminated drinking water on the microbiome and resistome of dairy cattle. We identified bacterial antimicrobial resistance (AMR) genes in the feces, rumen fluid, and nasopharynx of 16 dairy cattle 4 years after the environmental disaster. Cattle had been continuously exposed to heavy metal contaminated water until sample collection (A) and compared them to analogous samples from 16 dairy cattle in an unaffected farm, 356 km away (B). The microbiome and resistome of farm A and farm B differed in many aspects. The distribution of genes present in the cattle’s nasopharynx, rumen, and feces conferring AMR was highly heterogeneous, and most genes were present in only a few samples. The relative abundance and prevalence (presence/absence) of AMR genes were higher in farm A than in farm B. Samples from farm A had a higher prevalence (presence) of genes conferring resistance to multiple drugs, metals, biocides, and multi-compound resistance. Fecal samples had a higher relative abundance of AMR genes, followed by rumen fluid samples, and the nasopharynx had the lowest relative abundance of AMR genes detected. Metagenome functional annotation suggested that selective pressures of heavy metal exposure potentially skewed pathway diversity toward fewer, more specialized functions. This is the first study that evaluates the consequences of a Brazilian environmental accident with mining ore dam failure in the microbiome of dairy cows. Our findings suggest that the long-term persistence of heavy metals in the environment may result in differences in the microbiota and enrichment of antimicrobial-resistant bacteria. Our results also suggest that AMR genes are most readily detected in fecal samples compared to rumen and nasopharyngeal samples which had relatively lower bacterial read counts. Since heavy metal contamination has an effect on the animal microbiome, environmental management is warranted to protect the food system from hazardous consequences.
登录
查看更多内容
影响因子：
4.2
作者：
Holman DB;Timsit E;Amat S;Abbott DW;Buret AG;Alexander TW
通讯作者：
Alexander TW
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
DOI：
10.1098/rspa.1937.0109
发表时间：
1937-05-01
影响因子：
--
作者：
Bartlett, MS
通讯作者：
Bartlett, MS
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.7
作者：
Jami E;White BA;Mizrahi I
通讯作者：
Mizrahi I