In vivo imaging of epileptic foci in rats using a miniature probe integrating diffuse optical tomography and electroencephalographic source localization.
In vivo imaging of epileptic foci in rats using a miniature probe integrating diffuse optical tomography and electroencephalographic source localization.
复制标题
作者：
Yang H;Zhang T;Zhou J;Carney PR;Jiang H
本工作的目标是建立一种新的双模态脑映射技术的基础上扩散光学断层扫描（DOT）和脑电图源定位（ESL），可以长期/颅内记录光学/EEG数据，以精确地映射癫痫发作和定位癫痫发作区和相关的癫痫脑网络。采用双模式成像系统对实验性急性局灶性癫痫大鼠模型中的发作进行成像。在ESL中，利用DOT的深度信息作为约束条件，增强了图像的重建。根据不同位置或不同深度的癫痫灶定位，对动物组进行比较。这种新的成像技术成功地定位了1 mm，2 mm和3 mm深度注射甲碘荷包牡丹碱诱发的大鼠癫痫发作区。结果表明，从DOT的深度信息纳入ESL图像重建导致更准确和可靠的ESL图像。虽然ESL图像显示源定位的水平移位，但DOT准确地识别了发作病灶。在一种情况下，当在DOT/ESL接口的视场（FOV）之外的部位处注射BMI时，ESL给出病灶的假阳性检测，而DOT示出阴性检测。这项研究首次使用植入式DOT识别癫痫发作区。此外，DOT/ESL的组合从未在神经科学和癫痫成像中被记录。这项技术将使我们能够精确地测量神经活动和血液动力学反应在完全相同的组织部位，并在皮层和皮层下的水平。
The goal of this work is to establish a new dual-modal brain mapping technique based on diffuse optical tomography (DOT) and electroencephalographic source localization (ESL) that can chronically/intracranially record optical/EEG data to precisely map seizures and localize the seizure onset zone and associated epileptic brain network. The dual-modal imaging system was employed to image seizures in an experimental acute bicuculline methiodide rat model of focal epilepsy. Depth information derived from DOT was used as constraint in ESL to enhance the image reconstruction. Groups of animals were compared based on localization of seizure foci, either at different positions or at different depths. This novel imaging technique successfully localized the seizure onset zone in rat induced by bicuculline methiodide injected at a depth of 1mm, 2mm and 3mm, respectively. The results demonstrated that the incorporation of the depth information from DOT into the ESL image reconstruction resulted in more accurate and reliable ESL images. Although the ESL images showed a horizontal shift of the source localization, the DOT identified the seizure focus accurately. In one case, when the BMI was injected at a site outside the field of view (FOV) of the DOT/ESL interface, ESL gives false positive detection of the focus while DOT shows negative detection. This study represents the first to identify seizure onset zone using implantable DOT. In addition, the combination of DOT/ESL has never been documented in neuroscience and epilepsy imaging. This technology will enable us to precisely measure the neural activity and hemodynamic response at exactly the same tissue site and at both cortical and sub cortical levels.
登录
查看更多内容
影响因子：
5.7
作者：
Rullmann M;Anwander A;Dannhauer M;Warfield SK;Duffy FH;Wolters CH
通讯作者：
Wolters CH
DOI：
10.1016/s0013-4694(97)00115-6
发表时间：
1998-02-01
期刊：
ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY
影响因子：
--
作者：
Koles, ZJ
通讯作者：
Koles, ZJ
影响因子：
5.7
作者：
Keeser, D.;Padberg, F.;Mulert, C.
通讯作者：
Mulert, C.
影响因子：
4.8
作者：
Duempelmann, Matthias;Ball, Tonio;Schulze-Bonhage, Andreas
通讯作者：
Schulze-Bonhage, Andreas
影响因子：
5.6
作者：
AWAD, IA;ROSENFELD, J;LUDERS, H
通讯作者：
LUDERS, H