Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni.
Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni.
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DOI：
10.1016/j.molbiopara.2008.08.004
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发表时间：
2008-12
影响因子：
1.5
通讯作者：
Cunningham, Charles
中科院分区：
文献类型：
--
作者：
Aragon, Anthony D.;Imani, Reza A.;Blackburn, Vint R.;Cunningham, Charles
身体对病原体感染的防御可以采取多种形式。例如，在发生急性血吸虫病时，患者通常会发热，同时伴有幼虫成熟、迁移和早期产卵。随着感染的建立，寄生虫受到宿主免疫系统产生的氧化应激。血吸虫病最常见的治疗方法是抗蠕虫药物吡喹酮。然而，其有效性是有限的，因为它不能杀死感染后2 - 4周的寄生虫。显然，需要新的抗β-内酰胺酶药物。我们假设，基因产物表达的一部分，对热和/或氧化应激的保护性反应是未来药物开发的潜在治疗靶点。利用12，166个元件的寡核苷酸芯片来表征热和氧化应激诱导的曼氏血吸虫基因，我们发现1，878个S。热胁迫显著诱导了mansoni元素的产生。这些包括先前报道的表达HSP 40、HSP 70和HSP 86同源物的热休克基因。氧化应激诱导了1001种元素，包括表达超氧化物歧化酶、谷胱甘肽过氧化物酶和乙醛脱氢酶的同源物。72个元素是共同的两种压力，并可能被利用在开发新的抗染色体治疗。
The body’s defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host immune system. The most common treatment for schistosomiasis is the anti-helminthic drug praziquantel. Its effectiveness, however, is limited due to its inability to kill schistosomes 2 – 4 weeks post-infection. Clearly there is a need for new antischistosomal drugs. We hypothesize that gene products expressed as part of a protective response against heat and/or oxidative stress are potential therapeutic targets for future drug development. Using a 12,166 element oligonucleotide microarray to characterize Schistosoma mansoni genes induced by heat and oxidative stress we found that 1,878 S. mansoni elements were significantly induced by heat stress. These included previously reported heat-shock genes expressing homologs of HSP40, HSP70 and HSP86. One thousand and one elements were induced by oxidative stress including those expressing homologs of superoxide dismutase, glutathione peroxidase and aldehyde dehydrogenase. Seventy-two elements were common to both stressors and could potentially be exploited in the development of novel anti-schistosomal therapeutics.
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影响因子：
12.3
作者：
Aragon AD;Quiñones GA;Thomas EV;Roy S;Werner-Washburne M
通讯作者：
Werner-Washburne M
影响因子：
1.2
作者：
Cross, John H.;Vieira, Paulo;da Costa, Jose M. Correia
通讯作者：
da Costa, Jose M. Correia
影响因子：
4.7
作者：
Ding, QX;Keller, JN
通讯作者：
Keller, JN
DOI：
10.1073/pnas.93.10.5116
发表时间：
1996-05-14
影响因子：
11.1
作者：
Davidson, JF;Whyte, B;Schiestl, RH
通讯作者：
Schiestl, RH
影响因子：
14.9
作者：
Flanagan, JF;Peterson, CL
通讯作者：
Peterson, CL