Gene-based mapping of trehalose biosynthetic pathway genes reveals association with source- and sink-related yield traits in a spring wheat panel.
Gene-based mapping of trehalose biosynthetic pathway genes reveals association with source- and sink-related yield traits in a spring wheat panel.
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DOI：
10.1002/fes3.292
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发表时间：
2021-08
影响因子：
5
通讯作者：
Paul MJ
中科院分区：
文献类型：
--
作者：
Lyra DH;Griffiths CA;Watson A;Joynson R;Molero G;Igna AA;Hassani-Pak K;Reynolds MP;Hall A;Paul MJ
海藻糖6磷酸（T6 P）信号调节碳的使用和分配，是提高作物产量的目标。然而，海藻糖磷酸合酶（TPS）和海藻糖磷酸磷酸酶（TPP）基因对源和库相关性状的具体贡献在很大程度上仍然未知。我们使用TPS和TPP基因的富集捕获测序来估计和划分春小麦（Triticum aestivum）育种面板中产量相关性状的遗传变异，该面板专门用于捕获75，000个CIMMYT小麦品种集合的多样性。12个表型与TPS和TPP基因的变异相关，包括株高和生物量（源），每穗小穗数，穗生长和籽粒灌浆性状（库），这表明积极和消极的基因选择的迹象。单个基因解释了生物量和谷物相关性状的遗传率比例。三个TPS 1同源物对性状变异特别重要。在TPS和TPP基因家族内部和之间发现了株高和籽粒相关性状的上位性相互作用。当基因效应与全基因组标记相结合时，基于基因的预测提高了粒重的预测能力。我们的研究已经产生了丰富的信息TPS和TPP基因的自然变异相关的产量潜力，这证实了T6 P在资源分配中的作用，并在影响性状，如粒数和大小，确认其他研究，现在开辟了利用自然遗传变异的可能性更广泛地更好地了解本地基因的贡献产量性状纳入育种计划。T6 P信号通路是资源分配和源库相互作用的中央调节系统，并且正在成为玉米、水稻、小麦和高粱等作物的重要靶点（Paul等人，2018年; Paul等人，2020年）。在这里，我们第一次分析了TPS和TPP基因的综合外显子组SNP信息，并在一个专门设计用于代表75，000个CIMMYT品系遗传多样性的春小麦小组中剖析了产量相关性状的遗传结构（Molero et al.，2019年）。这些数据显示TPS和TPP基因与农艺性状的显着关系，具有历史选择的证据，并确定了未来选择TPS和TPP基因和策略杂交以提高产量的机会。
Trehalose 6‐phosphate (T6P) signalling regulates carbon use and allocation and is a target to improve crop yields. However, the specific contributions of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) genes to source‐ and sink‐related traits remain largely unknown. We used enrichment capture sequencing on TPS and TPP genes to estimate and partition the genetic variation of yield‐related traits in a spring wheat (Triticum aestivum) breeding panel specifically built to capture the diversity across the 75,000 CIMMYT wheat cultivar collection. Twelve phenotypes were correlated to variation in TPS and TPP genes including plant height and biomass (source), spikelets per spike, spike growth and grain filling traits (sink) which showed indications of both positive and negative gene selection. Individual genes explained proportions of heritability for biomass and grain‐related traits. Three TPS1 homologues were particularly significant for trait variation. Epistatic interactions were found within and between the TPS and TPP gene families for both plant height and grain‐related traits. Gene‐based prediction improved predictive ability for grain weight when gene effects were combined with the whole‐genome markers. Our study has generated a wealth of information on natural variation of TPS and TPP genes related to yield potential which confirms the role for T6P in resource allocation and in affecting traits such as grain number and size confirming other studies which now opens up the possibility of harnessing natural genetic variation more widely to better understand the contribution of native genes to yield traits for incorporation into breeding programmes. The T6P signalling pathway is a central regulatory system of resource allocation and source‐sink interactions and is emerging as an important target in crops such as maize, rice, wheat and sorghum (Paul et al., 2018; Paul et al., 2020). Here, for the first time, we analysed comprehensive exome SNP information for TPS and TPP genes and dissected the genetic architecture of yield‐related traits in a spring wheat panel specially designed to represent the genetic diversity of 75,000 CIMMYT lines (Molero et al., 2019). The data showed significant relationships of TPS and TPP genes with agronomic traits with evidence of historical selection and identified opportunities for future selection of TPS and TPP genes and strategic crossing for yield improvement.
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影响因子：
13.8
作者：
Allen AM;Winfield MO;Burridge AJ;Downie RC;Benbow HR;Barker GL;Wilkinson PA;Coghill J;Waterfall C;Davassi A;Scopes G;Pirani A;Webster T;Brew F;Bloor C;Griffiths S;Bentley AR;Alda M;Jack P;Phillips AL;Edwards KJ
通讯作者：
Edwards KJ
影响因子：
2.9
作者：
Ching A;Caldwell KS;Jung M;Dolan M;Smith OS;Tingey S;Morgante M;Rafalski AJ
通讯作者：
Rafalski AJ
影响因子：
3.3
作者：
Briggs, WH;Goldman, IL
通讯作者：
Goldman, IL
影响因子：
1.4
作者：
Barton, N. H.;Etheridge, A. M.;Veber, A.
通讯作者：
Veber, A.
影响因子：
56.9
作者：
Avni, Raz;Nave, Moran;Distelfeld, Assaf
通讯作者：
Distelfeld, Assaf