GPC1 Is Associated with Poor Prognosis and Treg Infiltration in Colon Adenocarcinoma.
GPC1 Is Associated with Poor Prognosis and Treg Infiltration in Colon Adenocarcinoma.
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DOI：
10.1155/2022/8209700
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发表时间：
2022
影响因子：
--
通讯作者：
Li, Ji-yu
中科院分区：
文献类型：
--
作者：
Liu, Ying;Ren, Hui;Yang, Mu-qing;Li, Ji-yu
Glypican-1 （GPC1）是一种糖基化蛋白，被认为是一种很有前景的癌症生物标志物。然而，GPC1在结肠腺癌（COAD）中的系统研究很少。我们基于癌症基因组图谱（The Cancer Genome Atlas， TCGA）进行生物信息学分析，并通过临床样本验证GPC1在结肠腺癌中过表达。Kaplan-Meier分析显示，GPC1高表达与较差的总生存期（OS）相关。Cox回归模型进一步显示GPC1表达是COAD的独立负预后因素。基因集富集分析表明，多种致癌信号通路在GPC1高表达与低表达的COAD肿瘤中存在差异富集，包括DNA甲基化、G2/M损伤检查点和端粒功能障碍。我们观察到GPC1的表达与免疫细胞浸润呈正相关，如调节性T细胞（Tregs）、巨噬细胞和肥大细胞，50个COAD组织的免疫组化显示GPC1的表达与Treg的富集呈正相关。我们的研究结果为预测COAD的预后提供了一个有希望的候选基因，并为肿瘤免疫提供了新的见解。需要进一步的研究来验证这些结果。
Glypican-1 (GPC1) is a glycosylated protein recognized as a promising biomarker for cancer. Nonetheless, there have been few systematic studies on GPC1 in colon adenocarcinoma (COAD). We conducted bioinformatic analysis based on The Cancer Genome Atlas (TCGA) and used clinical samples to verify that GPC1 is overexpressed in colon adenocarcinoma. Kaplan-Meier analysis showed that higher GPC1 expression was associated with poor overall survival (OS). The Cox regression model further showed that GPC1 expression is an independent negative prognostic factor for COAD. Gene set enrichment analysis demonstrated that multiple oncogenic signaling pathways were differentially enriched in GPC1 high- versus low-expressing COAD tumors, including DNA methylation, G2/M damage checkpoint, and telomere dysfunction. We observed a positive correlation between GPC1 expression and immune cell infiltration, such as regulatory T cells (Tregs), macrophages, and mast cells, and immunohistochemistry of 50 COAD tissues revealed that GPC1 expression was positively associated with Treg enrichment. Our results provide a promising candidate gene to predict the prognosis of COAD and new insights into tumor immunity. Further research is required to validate these results.
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