Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae.
Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae.
复制标题
酿酒酵母中核糖体蛋白 L11 结合蛋白 Rrs1p 突变和 5S rRNA 3' 延伸导致 60S 核糖体亚基组装的协同缺陷。
DOI：
10.1093/nar/gki772
复制
发表时间：
2005
影响因子：
14.9
通讯作者：
Mizuta, K
中科院分区：
文献类型：
--
作者：
Nariai, M;Tanaka, T;Okada, T;Shirai, C;Horigome, C;Mizuta, K
Rrs1p是一种核糖体蛋白l11结合蛋白，在60S核糖体亚基的生物发生中起重要作用。我们获得了带有rrs1-5突变的条件合成致死等位基因，并确定该突变位于编码外切酶的REX1中。高度保守的亮氨酸305在rex1-1中被色氨酸取代。rex1-1等位基因产生了3 '长5S rRNA。多体分析表明，rex1-1和rrs1-5在60S核糖体亚基的组装中引起了协同缺陷。体内和体外结合实验表明，Rrs1p与核糖体蛋白L5-5S rRNA复合物相互作用。rrs1-5突变减弱了Rrs1p与L5和L11的相互作用。这些数据表明L5-5S rRNA在60S核糖体亚基上的组装与L11通过Rrs1p的组装相协调。
Rrs1p, a ribosomal protein L11-binding protein, has an essential role in biogenesis of 60S ribosomal subunits. We obtained conditionally synthetic lethal allele with the rrs1-5 mutation and determined that the mutation is in REX1, which encodes an exonuclease. The highly conserved leucine at 305 was substituted with tryptophan in rex1-1. The rex1-1 allele resulted in 3′-extended 5S rRNA. Polysome analysis revealed that rex1-1 and rrs1-5 caused a synergistic defect in the assembly of 60S ribosomal subunits. In vivo and in vitro binding assays indicate that Rrs1p interacts with the ribosomal protein L5–5S rRNA complex. The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5–5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p.
登录
查看更多内容
影响因子：
2.9
作者：
SPIERER, P;ZIMMERMANN, RA
通讯作者：
ZIMMERMANN, RA
影响因子：
2.1
作者：
Tsujii, R;Miyoshi, K;Mizuta, K
通讯作者：
Mizuta, K
影响因子：
64.5
作者：
KOSHLAND, D;KENT, JC;HARTWELL, LH
通讯作者：
HARTWELL, LH
影响因子：
3.5
作者：
Miyoshi, K;Shirai, C;Mizuta, K
通讯作者：
Mizuta, K
影响因子：
4.8
作者：
Miyoshi, K;Tsujii, R;Mizuta, K
通讯作者：
Mizuta, K