Microarray analysis of distinct gene transcription profiles in non-eosinophilic chronic sinusitis with nasal polyps.
Microarray analysis of distinct gene transcription profiles in non-eosinophilic chronic sinusitis with nasal polyps.
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DOI：
10.2500/ajr.2008.22.3233
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发表时间：
2008-11
期刊：
影响因子：
--
通讯作者：
Steinke JW
中科院分区：
文献类型：
--
作者：
Payne SC;Han JK;Huyett P;Negri J;Kropf EZ;Borish L;Steinke JW
Recent literature has indicated the feasibility of microarray analysis in the characterization of chronic sinusitis. We hypothesized that previously unexplored inflammatory mechanisms would be involved in the pathophysiology of noneosinophilic chronic rhinosinusitis with nasal polyps (NE-CRSwNP) and that this technology could be used to identify the gene expression of these novel and previously known mediators. Patients with CRSwNP failing medical therapy were prospectively enrolled and NP tissue was removed at time of surgery. NE-CRSwNP was diagnosed based on clinical parameters including absence of allergic disease and confirmed with histopathology showing lack of eosinophilic infiltration. Messenger RNA (mRNA) transcripts extracted from study and control patients were then subjected to microarray analysis using Affymatrix based chips. Validation of findings was then confirmed via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Microarray analysis revealed activation of pathways involved in antigen presentation, cellular movement, hematopoiesis, carcinogenesis, apoptosis, and cell signaling. Previously unexplored genes of interest were identified and their differential regulation was validated via qRT-PCR. Our data showed up-regulation of innate inflammation genes (IL-6, IL-8, and monocyte chemoattractant protein 1), hypoxia-induced inflammation 1α, and fibrosis (tenascin) and lack of up-regulation of genes associated with allergic, eosinophilic inflammation (IL-4 and IL-13). Additionally, the genes for CXCL1 and autocrine motility factor receptor were novelly identified to be up-regulated This study explores the utility of gene microarray technology in identifying unexplored targets of immune dysregulation in NE-CRSwNP. Furthermore, the data characterize the immunologic profile of NE-CRSwNP as it differs from other forms of CRSwNP, in particular, those known to be associated with eosinophilic inflammation.
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DOI：
10.1016/j.otohns.2004.09.067
发表时间：
2004-12
期刊：
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery
影响因子：
--
作者：
Meltzer EO;Hamilos DL;Hadley JA;Lanza DC;Marple BF;Nicklas RA;Bachert C;Baraniuk J;Baroody FM;Benninger MS;Brook I;Chowdhury BA;Druce HM;Durham S;Ferguson B;Gwaltney JM Jr;Kaliner M;Kennedy DW;Lund V;Naclerio R;Pawankar R;Piccirillo JF;Rohane P;Simon R;Slavin RG;Togias A;Wald ER;Zinreich SJ;American Academy of Allergy, Asthma and Immunology;American Academy of Otolaryngic Allergy;American Academy of Otolaryngology-Head and Neck Surgery;American College of Allergy, Asthma and Immunology;American Rhinologic Society
通讯作者：
American Rhinologic Society
影响因子：
3.4
作者：
Kim, Jeong-Whun;Hong, Sung-Lyong;Rhee, Chae-Seo
通讯作者：
Rhee, Chae-Seo
影响因子：
2.6
作者：
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通讯作者：
Steinke, John W.
DOI：
10.2500/ajr.2007.21.2968
发表时间：
2007-03-01
期刊：
AMERICAN JOURNAL OF RHINOLOGY
影响因子：
--
作者：
Figueiredo, Claudia R.;Santos, Rodrigo P.;Weckx, Luc L. M.
通讯作者：
Weckx, Luc L. M.
影响因子：
6.1
作者：
Benson, M;Olsson, M;Cardell, LO
通讯作者：
Cardell, LO