Harnessing genetic potential of wheat germplasm banks through impact-oriented-prebreeding for future food and nutritional security.
Harnessing genetic potential of wheat germplasm banks through impact-oriented-prebreeding for future food and nutritional security.
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DOI：
10.1038/s41598-018-30667-4
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发表时间：
2018-08-21
影响因子：
4.6
通讯作者：
Pixley KV
中科院分区：
文献类型：
--
作者：
Singh S;Vikram P;Sehgal D;Burgueño J;Sharma A;Singh SK;Sansaloni CP;Joynson R;Brabbs T;Ortiz C;Solis-Moya E;Govindan V;Gupta N;Sidhu HS;Basandrai AK;Basandrai D;Ledesma-Ramires L;Suaste-Franco MP;Fuentes-Dávila G;Moreno JI;Sonder K;Singh VK;Singh S;Shokat S;Arif MAR;Laghari KA;Srivastava P;Bhavani S;Kumar S;Pal D;Jaiswal JP;Kumar U;Chaudhary HK;Crossa J;Payne TS;Imtiaz M;Sohu VS;Singh GP;Bains NS;Hall A;Pixley KV
外来小麦遗传资源在加速粮食产量增加方面的价值在很大程度上未经证实和实现。我们使用新一代测序技术，结合多环境表型分析，研究了外来基因组对984个三元杂交衍生（外来/elite 1//elite 2）预育种系（PBL）的贡献。单倍型图为基础的和SNP标记的方法，这些线的基因组特征显示外来的具体印记的16.1至25.1%，这与理论预期的25%相比。在基因库中鉴定的染色体6D上的频率为0.4%的罕见且有利的单倍型（GT）使热胁迫下的谷物产量（戈伊）损失最小化，而在灌溉条件下没有戈伊损失。更具体地说，单倍型GT的'T'等位基因起源于节节麦，并且在研究中使用的所有优良品系中不存在。SNP的计算机分析显示与Ae中的一个候选基因编码的α-还原酶IRL样蛋白的命中。tauschii还在染色体1A、6A和2B上鉴定了对非生物/生物胁迫有效的稀有单倍型。结果表明，外来种质的积极贡献PBLs来自CIMMYT的最好的精英线与外来杂交。这是CIMMYT的一项主要的以影响为导向的育种前工作，导致大规模开发PBL，用于在气候变化情景下解决粮食安全问题的育种计划。
The value of exotic wheat genetic resources for accelerating grain yield gains is largely unproven and unrealized. We used next-generation sequencing, together with multi-environment phenotyping, to study the contribution of exotic genomes to 984 three-way-cross-derived (exotic/elite1//elite2) pre-breeding lines (PBLs). Genomic characterization of these lines with haplotype map-based and SNP marker approaches revealed exotic specific imprints of 16.1 to 25.1%, which compares to theoretical expectation of 25%. A rare and favorable haplotype (GT) with 0.4% frequency in gene bank identified on chromosome 6D minimized grain yield (GY) loss under heat stress without GY penalty under irrigated conditions. More specifically, the ‘T’ allele of the haplotype GT originated in Aegilops tauschii and was absent in all elite lines used in study. In silico analysis of the SNP showed hits with a candidate gene coding for isoflavone reductase IRL-like protein in Ae. tauschii. Rare haplotypes were also identified on chromosomes 1A, 6A and 2B effective against abiotic/biotic stresses. Results demonstrate positive contributions of exotic germplasm to PBLs derived from crosses of exotics with CIMMYT’s best elite lines. This is a major impact-oriented pre-breeding effort at CIMMYT, resulting in large-scale development of PBLs for deployment in breeding programs addressing food security under climate change scenarios.
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影响因子：
30.8
作者：
Moore, John W.;Herrera-Foessel, Sybil;Lagudah, Evans
通讯作者：
Lagudah, Evans
影响因子：
2.3
作者：
COX, TS;RAUPP, WJ;GILL, BS
通讯作者：
GILL, BS
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
5.6
作者：
Qian L;Hickey LT;Stahl A;Werner CR;Hayes B;Snowdon RJ;Voss-Fels KP
通讯作者：
Voss-Fels KP
影响因子：
56.9
作者：
Gabriel, SB;Schaffner, SF;Altshuler, D
通讯作者：
Altshuler, D