A localized surface plasmon resonance imaging instrument for multiplexed biosensing.
A localized surface plasmon resonance imaging instrument for multiplexed biosensing.
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DOI：
10.1021/ac400192f
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发表时间：
2013-05-07
影响因子：
7.4
通讯作者：
Van Duyne, Richard P.
中科院分区：
文献类型：
--
作者：
Ruemmele, Julia A.;Hall, W. Paige;Ruvuna, Laura K.;Van Duyne, Richard P.
局域表面等离子体共振 (LSPR) 光谱已广泛用于表面相互作用的无标记、高灵敏度测量。 LSPR 成像 (LSPRi) 具有 LSPR 的全部优点，但通过同时探测单个样品表面上的多个可单独寻址的传感器来实现高通量、多重测量。每个空间上不同的传感器都可以定制，以提供有关不同表面功能或反应环境的数据。此前，LSPRi 专注于尺寸尺度非常小的单粒子传感。在这里，我们创建了定义的宏观纳米粒子阵列，这些阵列与常见的图案化方法（例如浸笔纳米光刻和多通道微流体输送装置）兼容。通过这种新的 LSPR 传感格式，我们报告了多重 LSPR 成像的首次演示，并表明我们仪器增加的吞吐量能够在单个传感器表面上收集完整的朗缪尔结合曲线。此外，单链 DNA 与固定在传感器表面的互补序列的杂交证明了多重 LSPR 传感器具有高度选择性。这项工作中描述的 LSPR 阵列表现出均匀的灵敏度和可定制的光学特性，使其成为对各种分子结合相互作用进行高通量、无标记分析的理想平台。
Localized surface plasmon resonance (LSPR) spectroscopy has been widely used for label-free, highly-sensitive measurements of interactions at a surface. LSPR imaging (LSPRi) has the full advantages of LSPR, but enables high-throughput, multiplexed measurements by simultaneously probing multiple individually addressable sensors on a single sample surface. Each spatially distinct sensor can be tailored to provide data regarding different surface functionalities or reaction environments. Previously, LSPRi has focused on single particle sensing where the size scale is very small. Here, we create defined macroscale arrays of nanoparticles that are compatible with common patterning methods such as dip-pen nanolithography and multi-channel microfluidic delivery devices. With this new LSPR sensing format, we report the first demonstration of multiplexed LSPR imaging and show that the increased throughput of our instrument enables the collection of a complete Langmuir binding curve on a single sensor surface. In addition, the multiplexed LSPR sensor is highly selective, as demonstrated by the hybridization of single-stranded DNA to complementary sequences immobilized on the sensor surface. The LSPR arrays described in this work exhibit uniform sensitivity and tailorable optical properties, making them an ideal platform for high-throughput, label-free analysis of a variety of molecular binding interactions.
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