A molecular mechanosensor for real-time visualization of appressorium membrane tension in Magnaporthe oryzae.
A molecular mechanosensor for real-time visualization of appressorium membrane tension in Magnaporthe oryzae.
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DOI：
10.1038/s41564-023-01430-x
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发表时间：
2023-08
影响因子：
28.3
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中科院分区：
文献类型：
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稻瘟病菌稻瘟病菌利用一种被称为附着胞的受压感染细胞驱动一根坚硬的穿透钉穿过叶片角质层。附着胞巨大的内部压力是非常具有挑战性的研究，使我们对植物感染的细胞力学的理解不完整。在这里，利用膜靶向分子机械探针的荧光寿命成像，我们量化了m.o ryzae中膜张力的变化。我们发现，附着胞中的极端压力导致膜力学中的大规模空间异质性，远远大于之前在任何细胞类型中观察到的异质性。相比之下，非致病性黑色素缺陷突变体表现出较低的空间均匀膜张力。传感器激酶∆sln1突变体在附着胞膨胀过程中表现出明显更高的膜张力，这表明sln1在整个植物感染过程中控制着膨胀。因此，这种非侵入性的活细胞成像技术为病原真菌入侵宿主的巨大侵入力提供了新的见解，为新的疾病干预策略提供了潜力。稻瘟病菌利用被称为附着胞的受压感染细胞物理破坏寄主植物角质层。膜靶向分子力学探针用于定量极端压力下附着胞膜张力的变化。
The rice blast fungus Magnaporthe oryzae uses a pressurized infection cell called an appressorium to drive a rigid penetration peg through the leaf cuticle. The vast internal pressure of an appressorium is very challenging to investigate, leaving our understanding of the cellular mechanics of plant infection incomplete. Here, using fluorescence lifetime imaging of a membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension in M. oryzae. We show that extreme pressure in the appressorium leads to large-scale spatial heterogeneities in membrane mechanics, much greater than those observed in any cell type previously. By contrast, non-pathogenic melanin-deficient mutants, exhibit low spatially homogeneous membrane tension. The sensor kinase ∆sln1 mutant displays significantly higher membrane tension during inflation of the appressorium, providing evidence that Sln1 controls turgor throughout plant infection. This non-invasive, live cell imaging technique therefore provides new insight into the enormous invasive forces deployed by pathogenic fungi to invade their hosts, offering the potential for new disease intervention strategies. Magnaporthe oryzae uses pressurized infection cells called appressoria to physically break the host plant cuticle. A membrane-targeting molecular mechanoprobe is used to quantify changes in membrane tension in appressoria under extreme pressure.
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影响因子：
14.8
作者：
Kagan VE;Mao G;Qu F;Angeli JP;Doll S;Croix CS;Dar HH;Liu B;Tyurin VA;Ritov VB;Kapralov AA;Amoscato AA;Jiang J;Anthonymuthu T;Mohammadyani D;Yang Q;Proneth B;Klein-Seetharaman J;Watkins S;Bahar I;Greenberger J;Mallampalli RK;Stockwell BR;Tyurina YY;Conrad M;Bayır H
通讯作者：
Bayır H
影响因子：
11.6
作者：
Lopez-Berges, Manuel S.;Rispail, Nicolas;Di Pietro, Antonio
通讯作者：
Di Pietro, Antonio
影响因子：
64.8
作者：
Harris CR;Millman KJ;van der Walt SJ;Gommers R;Virtanen P;Cournapeau D;Wieser E;Taylor J;Berg S;Smith NJ;Kern R;Picus M;Hoyer S;van Kerkwijk MH;Brett M;Haldane A;Del Río JF;Wiebe M;Peterson P;Gérard-Marchant P;Sheppard K;Reddy T;Weckesser W;Abbasi H;Gohlke C;Oliphant TE
通讯作者：
Oliphant TE
影响因子：
56.9
作者：
Bechinger, C;Giebel, KF;Bastmeyer, M
通讯作者：
Bastmeyer, M
影响因子：
4.8
作者：
Garcia-Saez, Ana J.;Chiantia, Salvatore;Schwille, Petra
通讯作者：
Schwille, Petra