Controlled self-assembly of alginate microgels by rapidly binding molecule pairs.
Controlled self-assembly of alginate microgels by rapidly binding molecule pairs.
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DOI：
10.1039/c7lc00500h
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发表时间：
2017-07-11
期刊：
影响因子：
6.1
通讯作者：
Mooney DJ
中科院分区：
文献类型：
--
作者：
Hu Y;Mao AS;Desai RM;Wang H;Weitz DA;Mooney DJ
细胞包封的微尺度聚合物水凝胶（微凝胶）的受控自组装在各种组织工程和再生医学应用中可能是有利的。本文探索了一种通过化学修饰海藻酸盐聚合物与结合对分子（BPM）组装的方法。用几种类型的BPM，特别是生物素和链霉亲和素和点击化学化合物修饰藻酸盐，并使用微流控平台制备成25-30 μm的微凝胶。这些微凝胶被证明在生理条件下自组装。通过结合互补的微凝胶在一个高的比例，大小定义的组合，创建和BPM类型和组装方法上的微凝胶的数量，每个集合和包装密度的影响进行了测定。此外，开发了一种磁性过程，以分离组装从单个微凝胶，并允许形成多层球状体。最后，将细胞单独封装到藻酸盐微凝胶中，并使用BPM修饰的藻酸盐组装，这表明在再生医学中的潜在应用。我们开发了一种自组装25至30微米大小的海藻酸盐微凝胶的方法，并证明其适用于单囊化干细胞和多层球体。
Controlled self-assembly of cell-encapsulating microscale polymeric hydrogels (microgels) could be advantageous in a variety of tissue engineering and regenerative medicine applications. Here, a method of assembly by chemical modification of alginate polymer with binding pair molecules (BPM) was explored. Alginate was modified with several types of BPM, specifically biotin and streptavidin and click chemistry compounds, and fabricated into 25–30-μm microgels using a microfluidic platform. These microgels were demonstrated to self-assemble under physiological conditions. By combining complementary microgels at a high ratio, size-defined assemblages were created, and the effects of BPM type and assembly method on the number of microgels per assemblage and packing density were determined. Furthermore, a magnetic process was developed to separate assemblages from single microgels, and allow formation of multilayer spheroids. Finally, cells were singly encapsulated into alginate microgels and assembled using BPM-modified alginate, suggesting potential applications in regenerative medicine. We develop a method for self-assembly of 25- to 30-um sized alginate microgels, and demonstrate its applicability for singly-encapsulated stem cells and multilayered spheroids.
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影响因子：
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