Alterations in Membrane Phospholipid Fatty Acids of Gram-Positive Piezotolerant Bacterium Sporosarcina sp. DSK25 in Response to Growth Pressure
Alterations in Membrane Phospholipid Fatty Acids of Gram-Positive Piezotolerant Bacterium Sporosarcina sp. DSK25 in Response to Growth Pressure
复制标题
革兰氏阳性耐压细菌孢子八叠球菌膜磷脂脂肪酸的改变。
DOI：
10.1007/s11745-014-3878-7
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发表时间：
2014-03
期刊：
影响因子：
1.9
通讯作者：
Svetlana A. Golovko
中科院分区：
文献类型：
--
作者：
Jiani Wang;Jiasong Fang;Jiangtao Li;Shamik Dasgupta;Li Zhang;Mikhail Y. Golovko;Svetlana A. Golovko
压力是海洋和深层生物圈的一个重要热力学性质，影响微生物的生理和生物化学。在这里，我们报告了我们对革兰氏阳性耐压细菌Sporosarcina sp. DSK 25对静水压力的反应的研究。菌株DSK 25以适应性的方式响应于生长压力的上移，并表现出磷脂脂肪酸的系统变化。随着压力从0.1 MPa（兆帕）增加到10 MPa（兆帕），DSK 25中的不饱和脂肪酸从总脂肪酸的21.7%增加到31.1%，而异支化和反异支化脂肪酸的水平保持不变。在较高的压力（30、50和60 MPa）下，不饱和脂肪酸的量减少，反异构化脂肪酸的量以异构化脂肪酸为代价从34.4%增加到49.9%。在DSK 25中首次发现了两种多不饱和脂肪酸（PUFA），18：2n-6和18：2n-x，后者的丰度比前者高得多。PUFA的浓度随着生长压力的增加而增加。这些结果表明，不饱和和甲基支链脂肪酸参与调节细菌膜流动性和环境相关参数（压力）的功能。耐压细菌Sporosarcina sp. DSK 25似乎利用两种调节机制来适应高压，一种是瞬时规模的快速响应机制，表现为单不饱和脂肪酸的生物合成增加，另一种是反异构化和多不饱和脂肪酸合成增加的长期适应机制。我们的研究结果进一步表明，革兰氏阳性嗜压细菌在适应高压方面与革兰氏阴性细菌的反应不同。
Pressure is an important thermodynamic property of the ocean and the deep biosphere that affects microbial physiology and biochemistry. Here, we report on our investigation of the response of Gram‐positive piezotolerant bacterium Sporosarcina sp. DSK25 to hydrostatic pressure. Strain DSK25 responded in an adaptive manner to upshifts of growth pressure and showed systematic changes in phospholipid fatty acids. As the pressure increased from 0.1 to 10 MPa (Megapascal), unsaturated fatty acids in DSK25 increased from 21.7 to 31.1 % of total fatty acids, while the level of iso‐ and anteiso‐branched fatty acids remained unchanged. At higher pressures (30, 50, and 60 MPa), the amount of unsaturated fatty acids decreased, and that of anteiso‐branched fatty acids increased from 34.4 to 49.9 % at the expense of iso‐branched fatty acids. For the first time, two polyunsaturated fatty acids (PUFA), 18:2n‐6 and 18:2n‐x, with the latter having much higher abundance than the former, were identified in DSK25. The concentration of the PUFA increased with growth pressure. These results indicate the involvement of unsaturated and methyl‐branched fatty acids in the modulation of bacteria membrane fluidity and function over environmentally relevant parameter (pressure). Piezotolerant bacterium Sporosarcina sp. DSK25 appears to utilize two regulatory mechanisms for adaptation to high pressure, a rapid‐responding mechanism on transient scale, expressed as increased biosynthesis of monounsaturated fatty acids, and a long‐term adaptation mechanism in increased synthesis of anteiso‐branched and polyunsaturated fatty acids. Our results further suggest that Gram‐positive piezophilic bacteria respond differently than Gram‐negative bacteria in adaptation to high pressure.
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DOI：
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发表时间：
1984
期刊：
--
影响因子：
--
作者：
M. Sinensky;A. Cossins
通讯作者：
M. Sinensky;A. Cossins
DOI：
10.1016/s0952-3278(02)00269-7
发表时间：
2003-02
期刊：
Prostaglandins, leukotrienes, and essential fatty acids
影响因子：
--
作者：
M. C. Mansilla;P. Aguilar;D. Albanesi;L. Cybulski;S. Altabe;D. de Mendoza
通讯作者：
M. C. Mansilla;P. Aguilar;D. Albanesi;L. Cybulski;S. Altabe;D. de Mendoza
DOI：
--
发表时间：
1995-09
期刊：
--
影响因子：
--
作者：
C. Kato;Seiko Suzuki;S. Hata;Takashi Ito;K. Horikoshi
通讯作者：
C. Kato;Seiko Suzuki;S. Hata;Takashi Ito;K. Horikoshi
DOI：
10.1073/pnas.95.12.6578
发表时间：
1998-06-09
影响因子：
11.1
作者：
Whitman, WB;Coleman, DC;Wiebe, WJ
通讯作者：
Wiebe, WJ
DOI：
10.1016/b978-0-323-85718-5.00004-2
发表时间：
2022
期刊：
Human Biochemistry
影响因子：
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作者：
G. Litwack
通讯作者：
G. Litwack